Summary of Study ST002198
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001401. The data can be accessed directly via it's Project DOI: 10.21228/M8MT48 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002198 |
| Study Title | Untargeted metabolomics of Pinus pinaster needles under heat and drought stress |
| Study Type | Untargeted MS-based metabolomics |
| Study Summary | Current projections for global climate change predict an increase in the intensity and frequency of heat waves and droughts. The improvement in our understanding of the mechanisms of how trees precisely can predict environmental threats and cope with these stresses benefits our natural selection or genetic improvement to the maintenance of forest sustainability. In this work, we investigate the metabolic changes in heat and drought combined stress in Pinus pinaster plantlets. Maritime pine is a coniferous tree with native populations distributed across the European Atlantic and Mediterranean basins and the north of Africa ranging from cool moist to warm dry climates. This species shows high plasticity and a contrasting adaptive capacity and resilience. This plasticity in the response to stress exposure may be associated with a differential ability to modulate their secondary metabolism. For this reason, the current study aims to investigate the gradual and synergetic metabolomic response using liquid chromatography coupled to mass spectrometry (LC-MS) based on untargeted metabolomic profiling of four stress levels. These metabolic profiles were supported by physiological and biochemical determinations. Our results showed that the metabolic profiles induced by low-stress exposition represent an adaptive conditioning mode with metabolome changes that help seedlings to cope with upcoming stress. The metabolism pathways involved in this response were mainly included in amino acid metabolism and carbohydrate metabolism leading to an enhanced accumulation of phenolics, flavonoids, and terpenoids. However, when the plantlets were exposed to higher-stress exposition, the secondary metabolites that starred the response are more complex and decorated, such as alkaloids, lignans, and glycosyloxyflavones. Those changes could help to maintain homeostasis and control the response magnitude on establishing and facilitating the plantlets’ survival. Overall, our findings provide new insights into the responsive mechanisms of the maritime pine under heat and drought stress in terms of metabolic profiles. |
| Institute | Universidad de Oviedo |
| Department | Department of Organisms and Systems Biology |
| Laboratory | Plant Physiology |
| Last Name | López Hidalgo |
| First Name | Cristina |
| Address | C/ Catedrático Rodrigo Uría s/n Oviedo 33071 |
| lopezhcristina@uniovi.es | |
| Phone | 985104774 |
| Submit Date | 2022-06-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-07-14 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR002296 |
| Treatment Summary: | The experimental layout was based on a factorial design with two factors: temperature and water availability. Before starting the experiment, plants were divided into two chambers for testing two temperatures (30 ºC and 40 ºC), which in turn were split again into two levels of water availability (“low-water-stress”, LWS, and “high-water-stress”, HWS). Consequently, four stress levels, 40 ºC-HWS, 40 ºC-LWS, 30 ºC-HWS, and 30 ºC-LWS were tested. In both chambers, twelve plants were divided into six pools of two plants (three for HWS and three for LWS). These pools of two plants were kept across the sampling and formed the three independent biological replicates analyzed for each stress level. |
| Treatment: | Heat and Drought |
| Treatment Dose: | High Water Stress and Low Water Stress in 30ºC and 40ºC. |
| Treatment Vehicle: | Fitoclima 1200, Aralab Ltd, Sintra, Portugal |
| Plant Growth Support: | Fitoclima 1200, Aralab Ltd, Sintra, Portugal |
| Plant Growth Location: | Oviedo, Asturias |
| Plant Plot Design: | Randomized design |
| Plant Light Period: | During this month, the plants in the growth chamber (Fitoclima 1200, Aralab Ltd, Sintra, Portugal) were kept at a light intensity of 400 µmol m−2·s−1 under long-day conditions (16 h light/8 h dark for photoperiod). |
| Plant Humidity: | Relative humidity (RH) were set to 25 ºC and 50 % RH during the day, and 15 ºC and 60 % RH during the night |
| Plant Temp: | 25ºC, 30ºC, and 40ºC |
| Plant Watering Regime: | Plants were well-watered to field capacity until soil dropped every two days. |
| Plant Nutritional Regime: | efore trial, seedlings had been acclimated over one month inside the chamber and were watered to field capacity with nutritive solution (N:P:K; 5:8:10). |
| Plant Growth Stage: | Two-year-old seedlings |
| Plant Metab Quench Method: | Liquid N2 |
| Plant Harvest Method: | Liquid N2 |
| Plant Storage: | Lyophilized |
