Summary of Study ST002384
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001534. The data can be accessed directly via it's Project DOI: 10.21228/M8DH62 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002384 |
Study Title | Lipidomics study of curcumin and epigallocatechin gallate on IgE-mediated degranulation of RBL-2H3 cells |
Study Summary | This study provides novel insights into the potential anti-allergic mechanism of food-derived curcumin and EGCG, and then facilitates the discovery of drug target and the development of diagnostic methods for allergic diseases. |
Institute | College of Marine Food and Biological Engineering, Jimei University |
Last Name | Yang |
First Name | Zhiqiang |
Address | No. 185 Yinjiang Road, Jimei District, Xiamen City, Fujian Province, China |
201911832016@jmu.edu.cn | |
Phone | 0592-6181487 |
Submit Date | 2022-07-19 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | LC-MS |
Release Date | 2022-12-27 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002479 |
Sampleprep Summary: | The MeOH/MTBE/H2O system was used for the extraction of lipid metabolites from cell samples. Cell culture dishes stored at -80 °C were first removed on ice and 1 mL of methanolic solution containing internal standards was added, consisting of 1 μg/mL FFA (C16:0)-d3 and FFA (C18:0)-d3, 0.87 μg/mL LPC (19:0) and SM (d18:1/12:0), 1.12 μg/mL PC (38: 0), 0.92 μg/mL PE (30:0), 0.67 μg/mL TG (45:0), and 0.47 μg/mL Cer (d18:1/17:0). The cells were transferred to 5 mL Eppendorf centrifuge tubes by scraping with a cell scraper, adding 2.5 mL of MTBE and vortexing for 30 s. The cells were further shaken for 30 min at 10 °C and 1000 rpm using a thermostatic mixer. 750 μL of ultrapure water was added, vortexed for 60 s and allowed to stand for 5 min, and then centrifuged at 12,000 g for 10 min at 6 °C. After separation of the two phases, 400 μL of the hydrophobic phase was collected and dried in a cryogenic vacuum centrifuge concentrator, and the resulting lyophilized sample was stored at -80 °C. |