Summary of Study ST002393
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001540. The data can be accessed directly via it's Project DOI: 10.21228/M8N138 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002393 |
| Study Title | Untargeted metabolomics analysis of DSS-treated murine intestinal microbiota cultivated in continuous bioreactors |
| Study Summary | We cultivated murine faecal microbiota in six replicate continuous bioreactors. Three bioreactors were supplemented with 1% DSS and compared to the untreated control bioreactors. |
| Institute | Helmholtz Centre for Environmental Research |
| Department | Molecular Systems Biology |
| Last Name | Engelmann |
| First Name | Beatrice |
| Address | Permoserstrasse 15, 04318 Leipzig, Germany |
| beatrice.engelmann@ufz.de | |
| Phone | +493411099 |
| Submit Date | 2022-11-28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-12-20 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002488 |
| Sampleprep Summary: | In brief, culture supernatants were thawed and 100 µL were mixed with 500 µL methanol:acetonitrile (ACN):water (2:3:1) followed by vortex and sonication. After centrifugation, 100 µL of the supernatant were dried in a SpeedVac™ vacuum concentrator (Eppendorf, Hamburg, Germany). Prior to measurement, the extract was resuspended in 100 µl 1 % ACN and 0.1 % formic acid in water. |
