Summary of Study ST002406
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001548. The data can be accessed directly via it's Project DOI: 10.21228/M8M42M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002406 |
| Study Title | Silicon ameliorates clubroot responses in canola (Brassica napus): A “multi-omics”-based investigation into possible mechanisms |
| Study Type | Timecourse experiment |
| Study Summary | Clubroot disease, caused by Plasmodiophora brassicae Woronin results in severe yield losses in Brassica crops, including canola. Silicon (Si) mitigates several stresses and enhances plant resistance to phytopathogens. We investigated the effects of Si on clubroot disease symptoms in canola at two concentrations of Si (Si1.0 and Si0.5). In addition, the effects of Si on P. brassicae-induced gene expression, endogenous levels of phytohormones and metabolites were also studied. Si application reduced clubroot symptoms and improved plant growth under greenhouse conditions. Pathogen-induced transcript-level changes were affected by Si treatment to P. brassicae with genes related to antioxidant activity, phytohormone biosynthesis and signalling, nitrogen metabolism and secondary metabolism exhibiting differential expression. Endogenous levels of several phytohormones (e.g., auxin, cytokinin, salicylic acid and abscisic acid), amino acids and secondary metabolites (e.g., glucosinolates) were affected by Si. This is the first report that Si ameliorates clubroot symptoms and its possible mode of action. |
| Institute | Trent University |
| Department | Biology |
| Laboratory | Emery Lab |
| Last Name | Kisiala |
| First Name | Anna |
| Address | 1600 West Bank Drive, Trent University, Peterborough, ON, K9L 0G2, Canada |
| annakisiala@trentu.ca | |
| Phone | 7057481011 |
| Submit Date | 2022-12-12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-01-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002501 |
| Sampleprep Summary: | A total of 27 samples from the three treatments (PC, Si0.5 and Si1.0) were used for metabolomic analyses at 7-, 14- and 21 dpi. Hi-Q seedlings were grown in the soil-mix as previously described with/without Si amendment, inoculated with P. brassicae, and subsequently, washed thoroughly, flash-frozen in liquid nitrogen and stored at -80°C until analysis. Each biological replicate was comprised of 5 individual plants per treatment, and three biological replicates were generated from three independent experiments. A total of 27 samples were generated for transcriptomics and metabolomics analyses [3 treatments (PC, Si0.5 and Si1.0) x 3 time-points (7-, 14- and 21 dpi) x 3 biological replicates]. |
| Sampleprep Protocol Filename: | Sample Prep protocol |
| Processing Storage Conditions: | -20℃ |
| Extraction Method: | 50% ACN |
| Extract Cleanup: | HLB SPE |
| Extract Storage: | -20℃ |
| Sample Resuspension: | Primary metabolites - 90% ACN, glucosinolates - 5% ACN |
| Sample Spiking: | Primary metabolites - Labeled amino acid mix; Glucosinolates - labeled CK mix |
