Summary of Study ST002406
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001548. The data can be accessed directly via it's Project DOI: 10.21228/M8M42M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002406 |
Study Title | Silicon ameliorates clubroot responses in canola (Brassica napus): A “multi-omics”-based investigation into possible mechanisms |
Study Type | Timecourse experiment |
Study Summary | Clubroot disease, caused by Plasmodiophora brassicae Woronin results in severe yield losses in Brassica crops, including canola. Silicon (Si) mitigates several stresses and enhances plant resistance to phytopathogens. We investigated the effects of Si on clubroot disease symptoms in canola at two concentrations of Si (Si1.0 and Si0.5). In addition, the effects of Si on P. brassicae-induced gene expression, endogenous levels of phytohormones and metabolites were also studied. Si application reduced clubroot symptoms and improved plant growth under greenhouse conditions. Pathogen-induced transcript-level changes were affected by Si treatment to P. brassicae with genes related to antioxidant activity, phytohormone biosynthesis and signalling, nitrogen metabolism and secondary metabolism exhibiting differential expression. Endogenous levels of several phytohormones (e.g., auxin, cytokinin, salicylic acid and abscisic acid), amino acids and secondary metabolites (e.g., glucosinolates) were affected by Si. This is the first report that Si ameliorates clubroot symptoms and its possible mode of action. |
Institute | Trent University |
Department | Biology |
Laboratory | Emery Lab |
Last Name | Kisiala |
First Name | Anna |
Address | 1600 West Bank Drive, Trent University, Peterborough, ON, K9L 0G2, Canada |
annakisiala@trentu.ca | |
Phone | 7057481011 |
Submit Date | 2022-12-12 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2023-01-04 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002507 |
Treatment Summary: | Sodium silicate (Na2SiO3, Mol. Wt: 122.06 g/L), obtained from Sigma-Aldrich (St. Louis, US), was mixed with 100 g Sunshine Professional Growing Mix (Sun Gro Horticulture, Seba Beach, Canada) and the following mixtures (treatments) were produced: 0.0 g Si (control), 0.1 g Si (Si:soil in 1:1000, wt/wt), 0.25 g (Si:soil in 1:400, wt/wt), 0.5 g (Si:soil in 1:200, wt/wt), 0.75 g (Si:soil in 3:400, wt/wt) and 1.0 g (Si:soil in 1:100, wt/wt). These treatments, hereafter, reported as Si0.0 or PC, Si0.1, Si0.25, Si0.5, Si0.75 and Si1.0, respectively. After mixing thoroughly, the pH of the soil mix was determined prior to seeding. A clubroot susceptible spring canola (Brassica napus L.) cultivar Hi-Q, developed at the University of Alberta, was used in this study. The plants were grown under greenhouse conditions (22/19°C and 16/8h photoperiod) on the above-mentioned six soil media (treatments) containing varying concentration of Si. A total of 30 plants, from three replications of each treatment (i.e. 10 plants per replication) were used. A single spore suspension of P. brassicae pathotype 3, classified as pathotype 3H (P3H) under the Canadian Clubroot Differential (CCD) set, was prepared by homogenizing the clubroot galls, and used to inoculate the seedlings following previously described procedures. |