Summary of Study ST002970

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001849. The data can be accessed directly via it's Project DOI: 10.21228/M8QB0V This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002970
Study TitleUntargeted metabolomics of fatty alcohol production at time 90 min of bioconversion
Study SummaryWe demonstrated applications of this system using three different engineered Escherichia coli requiring different cofactors (fatty alcohol, bioluminescence light, and alkane biosynthesis). The results showed that the XR/lactose system could enhance the production of each system by ~2-4-fold. Untargeted metabolomics and transcriptomics analyses revealed that XR only enhanced pathways related to specific cofactor utilization.
Institute
Vidyasirimedhi Institute of Science and Technology
Last NameSutthaphirom
First NameChalermroj
Address555, Moo 1, Payubnai, Wangchan, Rayong, 21210, Thailand
Emailchalermroj.s_s19@vistec.ac.th
Phone(+66)063-9639915
Submit Date2023-11-06
Num Groups2
Total Subjects8
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2023-11-20
Release Version1
Chalermroj Sutthaphirom Chalermroj Sutthaphirom
https://dx.doi.org/10.21228/M8QB0V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP003089
Sampleprep Summary:The pellets were resuspended in 2 mL of cold 100% methanol (-80˚C) (HPLC grade). The samples were frozen in liquid nitrogen and thawed on ice for 3 cycles for extracting intracellular metabolites. The suspensions were centrifuged at 18,800´g at -9˚C for 30 min. The supernatant was transferred into a new 15 mL falcon tube, frozen in liquid nitrogen, and freeze-dried at -80˚C, 1 mbar, for 16 hours. The dried samples were maintained on ice, resuspended in 500 µL of cold 50% ACN/water (HPLC grade), and centrifuged for 30 min at 18,800´g and at -9˚C. The supernatant was collected and stored at -80˚C until analyzing with mass spectrometry.
Processing Storage Conditions:On ice
Extraction Method:Cold methanol
Extract Storage:-80℃
Sample Resuspension:500 µL of 50% ACN/water
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