Summary of Study ST003031
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001884. The data can be accessed directly via it's Project DOI: 10.21228/M8672X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003031 |
Study Title | Early time-restricted eating improves markers of cardiometabolic health but has no impact on nutrient absorption in healthy adults |
Study Type | Randomized Controlled Trial |
Study Summary | Metabolomic analysis performed on 88 human plasma samples collected from 16 participants that received 2 treatments with 3 time points each. Samples were analyzed by UPLC-MS using a Waters Acquity UPLC and detected on a 4000 QTrap by multiple reaction monitoring (MRM) with negative mode electrospray ionization. |
Institute | California Polytechnic State University, San Luis Obispo |
Last Name | La Frano |
First Name | Michael |
Address | Cal Poly State University 1 Grand Avenue San Luis Obispo, CA 93407 |
mlafrano@calpoly.edu | |
Phone | (805) 756-6233 |
Submit Date | 2023-12-15 |
Num Groups | 2 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2024-01-23 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP003151 |
Sampleprep Summary: | Plasma (25 μL) was added to 1.5 mL Eppendorf tubes prior to the addition of 10 μL of 1 μM internal standard solution. Next, 750 μL chilled methanol was added and samples were vortexed 30 s before centrifugation at 15,000 × G for 10 min. Subsequently, the supernatant was collected and added to 1.5 mL high performance liquid chromatography (HPLC) amber glass vials, dried, and reconstituted in 100 μL 3:1 acetonitrile: methanol containing 1-cyclohexyl-ureido, 3-dodecanoic acid (CUDA; Sigma-Aldrich, St. Louis, MO, USA) solution. The solution was vortexed 30 s, transferred to microfilter tubes, and centrifuged at 10,000 × G for 3 min prior to transfer to a HPLC vial. |
Sampleprep Protocol Filename: | La_Frano_Lab_Methods_eTRE_v2.pdf |