Summary of Study ST003034

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001887. The data can be accessed directly via it's Project DOI: 10.21228/M8SX4V This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003034
Study TitleShort chain fatty acid (SCFA) analysis in a bioreactor model system exposed to PFAS and bisphenols.
Study SummaryAn in vitro bioreactor model system based on the simplified human microbiome model (SIHUMIx) was used to investigate the direct effects of either perfluorooctanoic acid (PFOA), perfluorohexanoic acid (PFHxA) and perfluorobutanoic acid (PFBA) or bisphenol S (BPS) and bisphenol F (BPF) or a combined mixture on the microbiota. To determine any changes in the main fermentation products of the bacterial cultures three short chain fatty acids (SCFAs) were analyzed, namely acetate butyrate and propionate. Only the PFAS exposed cultures showed a significant increase in the concentrations of acetate (KW-test P= 0.0431) and butyrate (KW-test P= 0.0158) during exposure. In none of the exposure cultures nor in the control did the concentration of propionate change significantly.
Institute
Helmholtz Centre for Environmental Research
DepartmentMolecular Systems Biology
Last NameEngelmann
First NameBeatrice
AddressPermoserstraße 15, Leipzipg, Saxony, 03418, Germany
Emailbeatrice.engelmann@ufz.de
Phone004934160251099
Submit Date2023-12-20
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-01-23
Release Version1
Beatrice Engelmann Beatrice Engelmann
https://dx.doi.org/10.21228/M8SX4V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP003154
Sampleprep Summary:In brief, 20 µl of each sample was mixed with ACN to a final concentration of 50 %. SCFAs were derivatized with 200 mM 3-nitrophenylhydrazine and 120 mM N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride in pyridine and then diluted in 10 % ACN.
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