Summary of Study ST001758

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001126. The data can be accessed directly via it's Project DOI: 10.21228/M8569X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001758
Study Title	Metabolomics of mouse cerebral cortical brain tissues comparing GF and CONV-R mice
Study Type	untargeted metabolomics
Study Summary	Using high coverage metabolomics, we profiled feces, blood sera and cerebral cortical brain tissues of germ-free C57BL/6 mice and their age-matched conventionally raised counterparts. Results revealed for all three sample matrices metabolomic signatures owing to microbiota, yielding hundreds of identified metabolites including 533 altered for feces, 231 for sera and 58 for brain tissues with numerous significantly enriched pathways involving aromatic amino acids and neurotransmitters.
Institute	University of North Carolina at Chapel Hill
Department	Department of Environmental Sciences and Engineering
Last Name	Lai
First Name	Yunjia
Address	1104 MHRC, 135 Dauer Dr., Chapel Hill, NC 27599
Email	yunjia.lai@outlook.com
Phone	9194805489
Submit Date	2021-04-28
Num Groups	2
Total Subjects	24
Num Males	12
Num Females	12
Publications	Nature Communications
Raw Data Available	Yes
Raw Data File Type(s)	mzXML
Analysis Type Detail	LC-MS
Release Date	2021-07-26
Release Version	1

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Treatment:

Treatment ID:	TR001848
Treatment Summary:	Steady states only: C57BL/6 mouse littermates of ~7 weeks old raised under GF and conventional conditions were age-matched upon selection for sacrifice and analyses, resulting in a final total of 24 mice, including 12 GF mice and 12 SPF CONV-R mice with 6 males and 6 females included in each. The animals were raised under the following conditions: 22ºC, 40-70% humidity, and a daily 12:12 hour light-dark cycle. For dietary administration, we consistently fed all mice the same purified (and sterile) standard rodent diets and provided tap water ad libitum.

Treatment ID:

TR001848

Treatment Summary:

Steady states only: C57BL/6 mouse littermates of ~7 weeks old raised under GF and conventional conditions were age-matched upon selection for sacrifice and analyses, resulting in a final total of 24 mice, including 12 GF mice and 12 SPF CONV-R mice with 6 males and 6 females included in each. The animals were raised under the following conditions: 22ºC, 40-70% humidity, and a daily 12:12 hour light-dark cycle. For dietary administration, we consistently fed all mice the same purified (and sterile) standard rodent diets and provided tap water ad libitum.