Summary of Study ST001499

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001015. The data can be accessed directly via it's Project DOI: 10.21228/M8H40X This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001499
Study TitleMetabolomics reveals the protective effect of isosteviol sodium against multiple organ injury in septic mice - Heart
Study SummarySepsis is a severe inflammatory disorder that can lead to multiple organ injury. Isosteviol sodium (STV-Na) is a terpenoid derived from stevioside that exerts anti-inflammatory, antioxidant and anticancer activities. However, the influence of STV-Na on sepsis remains unknown. Here, we assessed the potential effects of STV-Na on sepsis and multiple organ injury induced by lipopolysaccharide (LPS). We found that STV-Na increased the survival rate of mice treat with LPS, significantly improved the functions of the heart, lung, liver, and kidney, and reduced the production of inflammatory cytokines. Moreover, Multiorgan metabolomics analysis demonstrated that glutathione metabolism, purine metabolism, glycerophospholipid metabolism and pantothenate and CoA biosynthesis, were significantly altered by STV-Na. This study provides novel insights into the metabolite changes of multiple organ injury in septic mice, which may help characterize the underlying mechanism and provide an improved understanding of the therapeutic effects of STV-Na on sepsis. Mice are randomly assigned to 4 groups in study design. Control: saline + saline Model: saline + LPS; Treatment: STV-Na + LPS; Positive: dexamethasone (Dex) + LPS. Drugs were administered i.p. Six hours after LPS injection, mice were sacrificed. And blood and tissues (heart, lung, liver, spleen and kidney) were subjected to UHPLC-TIMS TOF MS/MS-based metabolomics analyses.
Institute
Guangdong University of Technology
Last NameWang
First NameShanping
AddressNo. 100, Waihuan Xilu, Guangzhou Higher Education Mega Center, Panyu District,
Emailshanpingwang@outlook.com
Phone15521002792
Submit Date2020-09-29
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2021-03-01
Release Version1
Shanping Wang Shanping Wang
https://dx.doi.org/10.21228/M8H40X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001015
Project DOI:doi: 10.21228/M8H40X
Project Title:Metabolomics reveals the protective effect of isosteviol sodium against multiple organ injury in septic mice
Project Summary:Sepsis is a severe inflammatory disorder that can lead to multiple organ injury. Isosteviol sodium (STV-Na) is a terpenoid derived from stevioside that exerts anti-inflammatory, antioxidant and anticancer activities. However, the influence of STV-Na on sepsis remains unknown. Here, we assessed the potential effects of STV-Na on sepsis and multiple organ injury induced by lipopolysaccharide (LPS). We found that STV-Na increased the survival rate of mice treat with LPS, significantly improved the functions of the heart, lung, liver, and kidney, and reduced the production of inflammatory cytokines. Moreover, Multiorgan metabolomics analysis demonstrated that glutathione metabolism, purine metabolism, glycerophospholipid metabolism and pantothenate and CoA biosynthesis, were significantly altered by STV-Na. This study provides novel insights into the metabolite changes of multiple organ injury in septic mice, which may help characterize the underlying mechanism and provide an improved understanding of the therapeutic effects of STV-Na on sepsis.
Institute:Guangdong University of Technology
Last Name:Wang
First Name:Shanping
Address:No. 100, Waihuan Xilu, Guangzhou Higher Education Mega Center, Panyu District,
Email:shanpingwang@outlook.com
Phone:15521002792

Subject:

Subject ID:SU001573
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Groups
SA126358Ctrl-8Control
SA126359Ctrl-1Control
SA126360Ctrl-7Control
SA126361Ctrl-6Control
SA126362Ctrl-2Control
SA126363Ctrl-4Control
SA126364Ctrl-3Control
SA126365Ctrl-5Control
SA126366LPS-7Model
SA126367LPS-8Model
SA126368LPS-6Model
SA126369LPS-5Model
SA126370LPS-1Model
SA126371LPS-3Model
SA126372LPS-2Model
SA126373LPS-4Model
SA126374Dex-4Positive
SA126375Dex-3Positive
SA126376Dex-1Positive
SA126377Dex-5Positive
SA126378Dex-8Positive
SA126379Dex-9Positive
SA126380Dex-2Positive
SA126381Dex-7Positive
SA126382Dex-6Positive
SA126383STV-2Treatment
SA126384STV-4Treatment
SA126385STV-3Treatment
SA126386STV-1Treatment
SA126387STV-5Treatment
SA126388STV-6Treatment
SA126389STV-8Treatment
SA126390STV-7Treatment
SA126391STV-9Treatment
Showing results 1 to 34 of 34

Collection:

Collection ID:CO001568
Collection Summary:Samples of blood were gathered in heparinized tubes and then spun down at 3000 rpm at 4 °C for 10 min to obtain supernatant of plasma samples for subsequent preparation. Heart, lung, liver, spleen and kidney samples were homogenized in a five-fold volume of normal saline, respectively, and spun down at 12,000 rpm at 4 °C for 20 min to acquire supernatants for further preparation.
Collection Protocol Filename:shanpingwang_Collection_Protocol.docx
Sample Type:Heart

Treatment:

Treatment ID:TR001588
Treatment Summary:After being acclimatized for 1 week, the mice were separated, at random, into four groups: 1) saline + saline; 2) saline + LPS; 3) STV-Na+ LPS; and 4) dexamethasone (Dex) + LPS. Mice were intraperitoneally administered saline (0.1 mL/10 g) or STV-Na (5, 10, 20 mg/kg) and Dex (10 mg/kg) two times per day every 12 h for 3 consecutive days, and one hour after the first intraperitoneal injection on day 3, saline (0.1 mL/g body weight) or LPS from E. coli (0111: B4, 20 mg/kg) was intraperitoneally administered.
Treatment Protocol Filename:shanpingwang_Treatment_Protocol.docx

Sample Preparation:

Sampleprep ID:SP001581
Sampleprep Summary:A total of 160 µL of MTBE solution (methyl-T-butyl-ether: methanol: water, 6/3/1, v/v/v) was applied to 40 µL of the plasma or tissue homogenate supernatant, vortexed for 30 min at 4°C and spun at 12,000 rpm for 30 min. Two extract components were produced: an organic hydrophobic layer and a hydrophilic layer. These two extracts were vacuum-dried and dissolved in 0.1% (v/v) formic acid in water (45 µL), followed by analysis. The pooled quality control (QC) samples including whole plasma and tissues were utilized for monitoring data acquisition performance throughout the analysis. Finally, 6 duplicate QC samples were prepared and injected at the start of the sequence, and after each of the six tissue samples was inserted, the QC samples were added to determine system stability.
Sampleprep Protocol Filename:shanpingwang_Sampleprep_protocol.docx
shanpingwang_Treatment_Protocol.docx

Combined analysis:

Analysis ID AN002482 AN002483
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters Acquity BEH C18 (50 x 2.1mm,1.7um) Waters Acquity BEH C18 (50 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Bruker timsTOF Bruker timsTOF
Ion Mode POSITIVE NEGATIVE
Units Intensity Intensity

Chromatography:

Chromatography ID:CH001819
Chromatography Summary:Chromatographic separations were conducted utilizing a Waters BEH C18, 2.1 mm×50 mm 1.7 µm particle column with a Dionex Ultimate 3000 UHPLC system from Thermo Fisher Scientific (CA, USA). The mobile phase encompassed water with 0.1% v/v formic acid (A) and acetonitrile with 0.1% v/v formic acid (B). Columns were kept at 40°C and eluted using a linear gradient: 2-30% B at 0-4 min, 30-40% B at 4-5 min, 40% B at 5-8 min, 40-60% B at 8-10 min, 60-100% B at 10-17 min, 100% B at 17-19 min, 100-2% B at 19-19.1 min, and 2% B at 19.1-25 min. To increase the amount of metabolites and save experimental time, a new sampling method was used to detect both the organic phase and the aqueous phase extracts (Qiuhui Xuan et al., 2018; Shanping Wang et al., 2019): 5 µL of organic phase extracts were first loaded without running the elution gradient, which lasted for one minute at the initial mobile phase, and then 5 µL of the aqueous phase extracts were added to the same column in order to start running the elution gradient using a 0.4 mL/min flow rate.
Methods Filename:shanpingwang_Chromatography_Methods.docx
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C18 (50 x 2.1mm,1.7um)
Column Temperature:40
Flow Rate:0.4 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS002302
Analysis ID:AN002482
Instrument Name:Bruker timsTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:MSMS Progenesis QI 2.1 software EZinfo 3.0 software
Ion Mode:POSITIVE
Analysis Protocol File:shanpingwang_Analysis_Protocol.docx
  
MS ID:MS002303
Analysis ID:AN002483
Instrument Name:Bruker timsTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:MSMS Progenesis QI 2.1 software EZinfo 3.0 software
Ion Mode:NEGATIVE
Analysis Protocol File:shanpingwang_Analysis_Protocol.docx
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