Summary of Study ST000056

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000053. The data can be accessed directly via it's Project DOI: 10.21228/M8C883 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000056
Study TitleEnvironmental impact on metabolomics and food allergy
Study TypeMetabolomics
Study SummaryThis metabolomics pilot and feasibility (P&F) was conducted to determine if varying the levels of environmental LPS exposure and immunization with the vaccine adjuvant alum alters host metabolism. Additionally, the metabolomics will identify biomarkers that can predict allergic disease development and or disease severity after a peanut challenge in hypersensitive mice. Information gained from the proposed studies may allow for the identification of individuals who are “at risk” or “not at risk” for the development of allergic disease. Furthermore, completion of these studies may lead to identification of biological targets that may be used to develop novel therapies to treat or prevent allergic disease via future funding.
Institute
University of North Carolina
DepartmentSystems and Translational Sciences
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2014-05-31
Num Groups2
Raw Data AvailableYes
Raw Data File Type(s)fid
Uploaded File Size68 M
Analysis Type DetailNMR
Release Date2015-06-01
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8C883
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000053
Project DOI:doi: 10.21228/M8C883
Project Title:Environmental impact on metabolomics and food allergy
Project Type:food allergy vaccine development
Project Summary:NIH R03 grant to develop a mouse model that evaluates the cumulative effect of reduced LPS exposure and vaccination with alum-adjuvanted vaccines on the development and severity of peanut allergy.
Institute:Duke University
Department:Medical Center
Last Name:Staats
First Name:Herman
Email:herman.staats@duke.edu
Phone:919-684-8823

Subject:

Subject ID:SU000075
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C3H/HeN
Gender:Female
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Bedding type Ovalbumin Immunization Peanut Sensitization
SA002845F_Clean_1582Clean Paper Bedding Alum + Ova + CpG (100 ug) Yes
SA002846F_Clean_1585Clean Paper Bedding Alum + Ova + CpG (100 ug) Yes
SA002847F_Clean_1584Clean Paper Bedding Alum + Ova + CpG (100 ug) Yes
SA002848F_Clean_1583Clean Paper Bedding Alum + Ova + CpG (100 ug) Yes
SA002849F_Clean_1581Clean Paper Bedding Alum + Ova + CpG (100 ug) Yes
SA002895F_Clean_1719Clean Paper Bedding Alum + Ova + CpG (100 ug) Yes
SA002896F_Clean_1718Clean Paper Bedding Alum + Ova + CpG (100 ug) Yes
SA002897F_Clean_1720Clean Paper Bedding Alum + Ova + CpG (100 ug) Yes
SA002898F_Clean_1717Clean Paper Bedding Alum + Ova + CpG (100 ug) Yes
SA002899F_Clean_1782Clean Paper Bedding Alum + Ova + CpG (50 ug) Yes
SA002900F_Clean_1784Clean Paper Bedding Alum + Ova + CpG (50 ug) Yes
SA002901F_Clean_1785Clean Paper Bedding Alum + Ova + CpG (50 ug) Yes
SA002902F_Clean_1781Clean Paper Bedding Alum + Ova + CpG (50 ug) Yes
SA002903F_Clean_1783Clean Paper Bedding Alum + Ova + CpG (50 ug) Yes
SA002850F_Clean_1590Clean Paper Bedding Alum + Ova + MPL Yes
SA002851F_Clean_1586Clean Paper Bedding Alum + Ova + MPL Yes
SA002852F_Clean_1588Clean Paper Bedding Alum + Ova + MPL Yes
SA002853F_Clean_1587Clean Paper Bedding Alum + Ova + MPL Yes
SA002854F_Clean_1589Clean Paper Bedding Alum + Ova + MPL Yes
SA002904F_Clean_1722Clean Paper Bedding Alum + Ova + MPL Yes
SA002905F_Clean_1723Clean Paper Bedding Alum + Ova + MPL Yes
SA002906F_Clean_1721Clean Paper Bedding Alum + Ova + MPL Yes
SA002907F_Clean_1725Clean Paper Bedding Alum + Ova + MPL Yes
SA002908F_Clean_1724Clean Paper Bedding Alum + Ova + MPL Yes
SA002855F_Clean_1579Clean Paper Bedding Alum + Ova Yes
SA002856F_Clean_1576Clean Paper Bedding Alum + Ova Yes
SA002857F_Clean_1580Clean Paper Bedding Alum + Ova Yes
SA002858F_Clean_1578Clean Paper Bedding Alum + Ova Yes
SA002859F_Clean_1577Clean Paper Bedding Alum + Ova Yes
SA002909F_Clean_1778Clean Paper Bedding Alum + Ova Yes
SA002910F_Clean_1713Clean Paper Bedding Alum + Ova Yes
SA002911F_Clean_1779Clean Paper Bedding Alum + Ova Yes
SA002912F_Clean_1711Clean Paper Bedding Alum + Ova Yes
SA002913F_Clean_1780Clean Paper Bedding Alum + Ova Yes
SA002914F_Clean_1714Clean Paper Bedding Alum + Ova Yes
SA002915F_Clean_1712Clean Paper Bedding Alum + Ova Yes
SA002916F_Clean_1777Clean Paper Bedding Alum + Ova Yes
SA002917F_Clean_1776Clean Paper Bedding Alum + Ova Yes
SA002860F_Clean_1599Clean Paper Bedding N/A N/A
SA002861F_Clean_1598Clean Paper Bedding N/A N/A
SA002862F_Clean_1600Clean Paper Bedding N/A N/A
SA002863F_Clean_1596Clean Paper Bedding N/A N/A
SA002864F_Clean_1597Clean Paper Bedding N/A N/A
SA002918F_Clean_1701Clean Paper Bedding N/A N/A
SA002919F_Clean_1704Clean Paper Bedding N/A N/A
SA002920F_Clean_1705Clean Paper Bedding N/A N/A
SA002921F_Clean_1702Clean Paper Bedding N/A N/A
SA002922F_Clean_1703Clean Paper Bedding N/A N/A
SA002865F_Clean_1595Clean Paper Bedding N/A Yes
SA002866F_Clean_1594Clean Paper Bedding N/A Yes
SA002867F_Clean_1592Clean Paper Bedding N/A Yes
SA002868F_Clean_1593Clean Paper Bedding N/A Yes
SA002869F_Clean_1591Clean Paper Bedding N/A Yes
SA002923F_Clean_1707Clean Paper Bedding N/A Yes
SA002924F_Clean_1708Clean Paper Bedding N/A Yes
SA002925F_Clean_1706Clean Paper Bedding N/A Yes
SA002926F_Clean_1709Clean Paper Bedding N/A Yes
SA002927F_Clean_1710Clean Paper Bedding N/A Yes
SA002870F_Dirty_492Dirty Corncob Bedding Alum + Ova + CpG (100 ug) Yes
SA002871F_Dirty_491Dirty Corncob Bedding Alum + Ova + CpG (100 ug) Yes
SA002872F_Dirty_494Dirty Corncob Bedding Alum + Ova + CpG (100 ug) Yes
SA002873F_Dirty_493Dirty Corncob Bedding Alum + Ova + CpG (100 ug) Yes
SA002874F_Dirty_495Dirty Corncob Bedding Alum + Ova + CpG (100 ug) Yes
SA002928F_Dirty_1742Dirty Corncob Bedding Alum + Ova + CpG (100 ug) Yes
SA002929F_Dirty_1741Dirty Corncob Bedding Alum + Ova + CpG (100 ug) Yes
SA002930F_Dirty_1744Dirty Corncob Bedding Alum + Ova + CpG (100 ug) Yes
SA002931F_Dirty_1743Dirty Corncob Bedding Alum + Ova + CpG (100 ug) Yes
SA002932F_Dirty_1793Dirty Corncob Bedding Alum + Ova + CpG (50 ug) Yes
SA002933F_Dirty_1792Dirty Corncob Bedding Alum + Ova + CpG (50 ug) Yes
SA002934F_Dirty_1791Dirty Corncob Bedding Alum + Ova + CpG (50 ug) Yes
SA002935F_Dirty_1795Dirty Corncob Bedding Alum + Ova + CpG (50 ug) Yes
SA002936F_Dirty_1794Dirty Corncob Bedding Alum + Ova + CpG (50 ug) Yes
SA002875F_Dirty_497Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002876F_Dirty_500Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002877F_Dirty_496Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002878F_Dirty_499Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002879F_Dirty_498Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002937F_Dirty_1748Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002938F_Dirty_1746Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002939F_Dirty_1749Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002940F_Dirty_1747Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002941F_Dirty_1750Dirty Corncob Bedding Alum + Ova + MPL Yes
SA002880F_Dirty_484Dirty Corncob Bedding Alum + Ova Yes
SA002881F_Dirty_481Dirty Corncob Bedding Alum + Ova Yes
SA002882F_Dirty_485Dirty Corncob Bedding Alum + Ova Yes
SA002883F_Dirty_483Dirty Corncob Bedding Alum + Ova Yes
SA002884F_Dirty_482Dirty Corncob Bedding Alum + Ova Yes
SA002942F_Dirty_1740Dirty Corncob Bedding Alum + Ova Yes
SA002943F_Dirty_1739Dirty Corncob Bedding Alum + Ova Yes
SA002944F_Dirty_1738Dirty Corncob Bedding Alum + Ova Yes
SA002945F_Dirty_1736Dirty Corncob Bedding Alum + Ova Yes
SA002946F_Dirty_1737Dirty Corncob Bedding Alum + Ova Yes
SA002947F_Dirty_1789Dirty Corncob Bedding Alum + Ova Yes
SA002948F_Dirty_1788Dirty Corncob Bedding Alum + Ova Yes
SA002949F_Dirty_1787Dirty Corncob Bedding Alum + Ova Yes
SA002950F_Dirty_1786Dirty Corncob Bedding Alum + Ova Yes
SA002951F_Dirty_1790Dirty Corncob Bedding Alum + Ova Yes
SA002885F_Dirty_490Dirty Corncob Bedding N/A N/A
SA002886F_Dirty_486Dirty Corncob Bedding N/A N/A
SA002887F_Dirty_489Dirty Corncob Bedding N/A N/A
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Collection:

Collection ID:CO000058
Collection Summary:-
Sample Type:feces

Treatment:

Treatment ID:TR000076

Sample Preparation:

Sampleprep ID:SP000071
Sampleprep Summary:Frozen fecal samples were transferred into numbered homogenizer bead tubes (ceramic) and diluted with D2O (Sigma-Aldrich, St. Louis, MO). Specimens with a mass of less than 100 mg were diluted with 0.5 mL; specimens with a mass of greater than 100 mg were diluted with 1.0 mL. The samples were then homogenized using the MagNA Lyser bead homogenizer for two 30 second pulses at 2000 rpm and then vortexed for an additional 30 seconds. The samples were pelleted by centrifugation at 12000 rcf for 5 minutes. 50 mg of extract were utilized for each specimen, and combined the fecal extract and D2O were a total volume of 630 µL. 70 µL of Chenomx ISTD solution into all tubes. Chenomx ISTD (Chenomx, Edmonton, Alberta, Canada) contains 5mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, chemical shift indicator), 100 mM imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) in D2O. 600 µL was transferred into each NMR tube for analysis.
Sampleprep Protocol Filename:RTI_Food_Allergy_Envirnomental_Metabolomics_Procedure.docx

Analysis:

Analysis ID:AN000094
Laboratory Name:RTI/DHMRI
Analysis Type:NMR
Acquisition Date:41724
Software Version:Topspin/ 3.0
Operator Name:Andrew Novokhatny, Kevin Knagge
Randomization Order:Yes
Detector Type:NMR
Data Format:fid, 1r
Chromatography ID:CH000062
Num Factors:15

NMR:

NMR ID:NM000026
Analysis ID:AN000094
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
NMR Comments:Intellegent binning with 50% looseness
Field Frequency Lock:Deuterium
Standard Concentration:0.5mM
Spectrometer Frequency:700 MHz
NMR Probe:cryogenically cooled ATMA inverse probe
NMR Solvent:10 % D2O
NMR Tube Size:5mm
Shimming Method:Gradient
Pulse Sequence:noesypr1d
Water Suppression:yes
Pulse Width:10.7 microseconds
Power Level:25.704 W
Receiver Gain:4.5
Offset Frequency:3296.0 Hz
Chemical Shift Ref Cpd:DSS
Temperature:25 degrees celsius
Number Of Scans:128
Dummy Scans:4
Acquisition Time:2.324 seconds
Relaxation Delay:2 s
Spectral Width:20.13580048
Num Data Points Acquired:65536
Real Data Points:65536
Line Broadening:0.5
Zero Filling:yes
Apodization:Lorentzian
Baseline Correction Method:Polynomial
Chemical Shift Ref Std:DSS
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