Summary of study ST000353

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000282. The data can be accessed directly via it's Project DOI: 10.21228/M8G308 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000353
Study TitleThe Development of Metabolomic Markers in African Bermudagrass (C. transvaalensis) for Sting Nematode (Belonolaimus longicaudatus) Response
Study TypeDisease response in terms of nematode reproduction and root weight
Study SummaryThe objective of the proposed pilot study is to identify metabolites up- and down-regulated in African bermudagrass that are tolerant and sensitive to the sting nematode and develop metabolomic markers for the highest expressed metabolites associated with tolerance. Future work will include additional accessions and species of bermudagrass, and testing under field conditions. Bermudagrass accessions identified as tolerant or sensitive by Pang et al. (2011) will be assessed under controlled greenhouse conditions to identify metabolites linked to sting nematode tolerance. Nematode response will be quantified through determination of root length and weight and the number of nematodes present 136 days after inoculation. Higher root length and weight indicate tolerance or resistance. Higher nematode counts indicate greater reproduction (i.e. a susceptible plant), while lower counts indicate that the accession may have some resistance. Metabolites from root tissue of these accessions will be compared to identify those associated with tolerance/resistance, and those that are associated with nematode infestation by comparing inoculated plants to uninoculated controls. Metabolomic markers will then be developed for the metabolites associated with tolerance/resistance. These markers will be used to guide future screening of bermudagrass accessions for breeding nematode-tolerant or -resistant varieties.
Institute
University of Florida
DepartmentSECIM
LaboratoryTurfgrass Breeding
Last NameBenda
First NameNicole
Address2005 SW 23rd St
Emailnbenda@ufl.edu
Phone352-792-4561
Submit Date2015-11-13
Num Groups3
Total Subjects42
Raw Data AvailableYes
Raw Data File Type(s).mzml
Analysis Type DetailLC-MS
Release Date2017-07-10
Release Version1
Nicole Benda Nicole Benda
https://dx.doi.org/10.21228/M8G308
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000282
Project DOI:doi: 10.21228/M8G308
Project Title:The Development of Metabolomic Markers in Bermudagrass (Cynodon spp.) for Sting Nematode (Belonolaimus longicaudatus) Response
Project Summary:The majority of golf courses in Florida experience damage from plant-parasitic nematodes and more than 80% of golf courses in Florida are at risk for nematode-related damage (Crow, 2005). With limited availability of nematicides and lack of tolerant cultivars, turf managers need new genetically resistant cultivars. Use of resistant or tolerant grasses is the most efficient, environmentally friendly and, in the long run, least costly component of IPM. Current screening of new genotypes for nematode resistance is labor- and time-intensive, with three months required soley for nematode development on test plants. We intend to develop metabolomic markers or a signature pattern associated with nematode resistance or tolerance. Screening plants for these markers or a signature pattern of would greatly speed up the screening process and development of resistant cultivars.
Institute:University of Florida
Department:Agronomy
Laboratory:Turfgrass Breeding
Last Name:Kenworthy
First Name:Kevin
Address:2005 SW 23rd Street, Gainesville FL 32611
Email:nbenda@ufl.edu
Phone:352-792-4561
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