Summary of Study ST000782

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000568. The data can be accessed directly via it's Project DOI: 10.21228/M87T1Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples  |  Perform analysis on untargeted data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST000782
Study TitleNuclear magnetic resonance-based metabolomics approach to evaluate the prevention effect of Camellia nitidissima Chi on colitis-associated carcinogenesis
Study TypeOriginal Research
Study SummaryColorectal cancer (CRC) is one of the most common malignant tumors worldwide, occurring in the colon or rectum portion of large intestine. With marked antioxidant, anti-inflammation and anti- tumor activities, Camellia nitidissima Chi has been used as an effective treatment of cancer. The azoxymethane/dextran sodium sulfate (AOM/DSS) induced CRC mice model was established and the prevention effect of Camellia nitidissima Chi extracts on the evolving of CRC was evaluated by gross examination, histopathological inspection, serum biochemistry analysis, combined with nuclear magnetic resonance (NMR)-based metabolomics and correlation network analysis. The results showed that Camellia nitidissima Chi extracts could significantly inhibit AOM/DSS induced CRC, relieve the colonic pathology and ameliorate the serum biochemistry, and could significantly reverse the disturbed metabolism towards the normal state. Moreover, the butanol fraction showed a better efficiency than the water-soluble fraction of Camellia nitidissima Chi. The study pave the way for further development of Camellia nitidissima Chi extracts as a potent CRC inhibitor. In this work, rats were divided into control group, carcinoma group, and two drug groups of JHC and CNC. The acronyms JHC means the water-soluble fraction of Camellia nitidissima Chi. The acronyms CNC means the butanol fraction of Camellia nitidissima Chi.
Institute
Nanjing University of Science & Technology
Last NameLi
First NameMinghui
Address200 Xiaolingwei Street, Nanjing 210094, China
Emailcpu_lmh@126.com
Phone+86 15952052370
Submit Date2017-05-21
Analysis Type DetailNMR
Release Date2017-10-03
Release Version1
Minghui Li Minghui Li
https://dx.doi.org/10.21228/M87T1Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000568
Project DOI:doi: 10.21228/M87T1Q
Project Title:Camellia nitidissima prevent colorectal cancer
Project Type:Nuclear magnetic resonance-based metabolomics
Project Summary:Colorectal cancer (CRC) is one of the most common malignant tumors worldwide, occurring in the colon or rectum portion of large intestine. With marked antioxidant, anti-inflammation and anti-tumor activities, Camellia nitidissima Chi has been used as an effective treatment of cancer. The azoxymethane/dextran sodium sulfate (AOM/DSS) induced CRC mice model was established and the prevention effect of Camellia nitidissima Chi extracts on the evolving of CRC was evaluated by gross examination, histopathological inspection, serum biochemistry analysis, combined with nuclear magnetic resonance (NMR)-based metabolomics and correlation network analysis. The results showed that Camellia nitidissima Chi extracts could significantly inhibit AOM/DSS induced CRC, relieve the colonic pathology and ameliorate the serum biochemistry, and could significantly reverse the disturbed metabolism towards the normal state. Moreover, the butanol fraction showed a better efficiency than the water-soluble fraction of Camellia nitidissima Chi. The study pave the way for further development of Camellia nitidissima Chi extracts as a potent CRC inhibitor.
Institute:Nanjing University of Science & Technology
Department:Department of Biological Engineering
Laboratory:Center for Molecular Metabolism
Last Name:Li
First Name:Minghui
Address:200 Xiaolingwei Str., Nanjing, Jiangsu, 210094, China
Email:cpu_lmh@126.com
Phone:+8615952052370

Subject:

Subject ID:SU000810
Subject Type:Animals
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animals; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Tissue Treatment
SA043035Intestine_Model1Intestine Carcinoma
SA043036Intestine_Model7Intestine Carcinoma
SA043037Intestine_Model6Intestine Carcinoma
SA043038Intestine_Model2Intestine Carcinoma
SA043039Intestine_Model3Intestine Carcinoma
SA043040Intestine_Model5Intestine Carcinoma
SA043041Intestine_Model4Intestine Carcinoma
SA043042Intestine_Model15Intestine Carcinoma
SA043043Intestine_Model16Intestine Carcinoma
SA043044Intestine_Model12Intestine Carcinoma
SA043045Intestine_Model11Intestine Carcinoma
SA043046Intestine_Model14Intestine Carcinoma
SA043047Intestine_Model13Intestine Carcinoma
SA043048Intestine_Model8Intestine Carcinoma
SA043049Intestine_Model9Intestine Carcinoma
SA043050Intestine_Model10Intestine Carcinoma
SA043026Intestine_CNC5Intestine CNC
SA043027Intestine_CNC3Intestine CNC
SA043028Intestine_CNC2Intestine CNC
SA043029Intestine_CNC6Intestine CNC
SA043030Intestine_CNC7Intestine CNC
SA043031Intestine_CNC9Intestine CNC
SA043032Intestine_CNC8Intestine CNC
SA043033Intestine_CNC1Intestine CNC
SA043034Intestine_CNC4Intestine CNC
SA043051Intestine_Control4Intestine Control
SA043052Intestine_Control1Intestine Control
SA043053Intestine_Control2Intestine Control
SA043054Intestine_Control3Intestine Control
SA043055Intestine_JHC4Intestine JHC
SA043056Intestine_JHC5Intestine JHC
SA043057Intestine_JHC3Intestine JHC
SA043058Intestine_JHC2Intestine JHC
SA043059Intestine_JHC1Intestine JHC
SA043060Intestine_JHC6Intestine JHC
SA043061Intestine_JHC7Intestine JHC
SA043062Intestine_JHC12Intestine JHC
SA043063Intestine_JHC10Intestine JHC
SA043064Intestine_JHC9Intestine JHC
SA043065Intestine_JHC11Intestine JHC
SA043066Intestine_JHC8Intestine JHC
SA043074Kideny_Model11Kidney Carcinoma
SA043075Kideny_Model10Kidney Carcinoma
SA043076Kideny_Model12Kidney Carcinoma
SA043077Kideny_Model14Kidney Carcinoma
SA043078Kideny_Model9Kidney Carcinoma
SA043079Kideny_Model13Kidney Carcinoma
SA043080Kideny_Model3Kidney Carcinoma
SA043081Kideny_Model1Kidney Carcinoma
SA043082Kideny_Model8Kidney Carcinoma
SA043083Kideny_Model2Kidney Carcinoma
SA043084Kideny_Model4Kidney Carcinoma
SA043085Kideny_Model7Kidney Carcinoma
SA043086Kideny_Model6Kidney Carcinoma
SA043087Kideny_Model5Kidney Carcinoma
SA043067Kideny_CNC7Kidney CNC
SA043068Kideny_CNC6Kidney CNC
SA043069Kideny_CNC3Kidney CNC
SA043070Kideny_CNC1Kidney CNC
SA043071Kideny_CNC5Kidney CNC
SA043072Kideny_CNC2Kidney CNC
SA043073Kideny_CNC4Kidney CNC
SA043088Kideny_Control2Kidney Control
SA043089Kideny_Control1Kidney Control
SA043090Kideny_Control3Kidney Control
SA043091Kideny_Control7Kidney Control
SA043092Kideny_Control4Kidney Control
SA043093Kideny_Control6Kidney Control
SA043094Kideny_Control5Kidney Control
SA043095Kideny_JHC12Kidney JHC
SA043096Kideny_JHC11Kidney JHC
SA043097Kideny_JHC2Kidney JHC
SA043098Kideny_JHC3Kidney JHC
SA043099Kideny_JHC1Kidney JHC
SA043100Kideny_JHC10Kidney JHC
SA043101Kideny_JHC5Kidney JHC
SA043102Kideny_JHC4Kidney JHC
SA043103Kideny_JHC9Kidney JHC
SA043104Kideny_JHC6Kidney JHC
SA043105Kideny_JHC8Kidney JHC
SA043106Kideny_JHC7Kidney JHC
SA043116Spleen_Model11Spleen Carcinoma
SA043117Spleen_Model10Spleen Carcinoma
SA043118Spleen_Model12Spleen Carcinoma
SA043119Spleen_Model14Spleen Carcinoma
SA043120Spleen_Model8Spleen Carcinoma
SA043121Spleen_Model15Spleen Carcinoma
SA043122Spleen_Model13Spleen Carcinoma
SA043123Spleen_Model9Spleen Carcinoma
SA043124Spleen_Model1Spleen Carcinoma
SA043125Spleen_Model7Spleen Carcinoma
SA043126Spleen_Model3Spleen Carcinoma
SA043127Spleen_Model2Spleen Carcinoma
SA043128Spleen_Model4Spleen Carcinoma
SA043129Spleen_Model5Spleen Carcinoma
SA043130Spleen_Model6Spleen Carcinoma
SA043107Spleen_CNC3Spleen CNC
SA043108Spleen_CNC2Spleen CNC
SA043109Spleen_CNC1Spleen CNC
SA043110Spleen_CNC4Spleen CNC
Showing page 1 of 2     Results:    1  2  Next     Showing results 1 to 100 of 126

Collection:

Collection ID:CO000804
Collection Summary:Briefly, tissues of intestines, kidneys and spleens weighting about 200 mg, 200 mg and 110 mg were weighted, homogenized with an icy cold solvent (the ratio of tissue weight to 50 % acetonitrile volume was 1: 5, w/v), vortexed, and centrifuged for 10 min at 12,000 g and 4 °C. The supernatant was transferred into fresh Eppendorf tube and was then frozen and lyophilized to dryness on a vacuum concentrator. The dried samples were stored at -80 °C until use.
Sample Type:Intestine

Treatment:

Treatment ID:TR000824
Treatment Summary:The animals were maintained under controlled conditions (22°C, 12h/12h 98 dark/light cycle). At the 15th week, there were 15 surviving mice in control group, 17 in model group, 14 in JHC group and 10 in CNC group, respectively. In this work, rats were divided into control group, carcinoma group, and two drug groups of JHC and CNC. The acronyms JHC means the water-soluble fraction of Camellia nitidissima Chi. The acronyms CNC means the butanol fraction of Camellia nitidissima Chi.

Sample Preparation:

Sampleprep ID:SP000817
Sampleprep Summary:For NMR measurements, samples were dissolved in 550 µL 99.8% deuteroxide (D2O) phosphate buffer (0.2 M, pH = 7.4) containing 0.05% (w/v) sodium 3-(trimethylsilyl) propionate-2, 2, 3, 3-d4 (TSP). After vortexing and centrifugation, the supernatant was then transferred to a 5 mm NMR tube for 1H NMR analysis.

Analysis:

Analysis ID:AN001238
Analysis Type:NMR
Num Factors:12

NMR:

NMR ID:NM000106
Analysis ID:AN001238
Instrument Name:Bruker
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:500 MHz
  logo