Summary of study ST001426

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000978. The data can be accessed directly via it's Project DOI: 10.21228/M88Q44 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001426
Study TitleDependence of the Staphylococcal Volatilome Composition on Microbial Nutrition
Study TypeUntargeted MS
Study SummaryIntroduction: In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, and selection of growth medium will substantially influence staph growth rates, genetic integrity, pathogenicity, and metabolic capacity. Few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. Objectives: The objective of this study was to determine the influence of rich media composition on the chemical characteristics of the volatilomes of Staphylococcus aureus and S. epidermidis. Methods: S. aureus (ATCC 12600) and S. epidermidis (ATCC 12228) were cultured in triplicate in four rich complex media (BHI, LB, MHB, and TSB), and the volatile metabolites produced by each culture were analyzed using headspace solid-phase microextraction combined with comprehensive two-dimensional gas chromatography – time-of-flight mass spectrometry (HS-SPME-GC×GC-TOFMS). Results: When comparing the chemical compositions of the staph volatilomes produced in each medium, we observed few differences when the presence versus absence of volatiles were compared. However, when the relative abundances of volatiles were included in the analyses, we observed that culturing staph in media containing free glucose (BHI and TSB) resulted in volatilomes dominated by acids and esters (67%). The low-glucose media (LB and MHB) produced ketones in greatest relative abundances, but the volatilome compositions in these two media were highly dissimilar. Conclusion: The staphylococcal volatilome is strongly influenced by the nutritional composition of the growth medium, especially the availability of free glucose, which is much more evident when the relative abundances of the volatiles are analyzed, compared to the presence versus absence.
Arizona State University
DepartmentSchool of Life Sciences
LaboratoryHeather D. Bean Lab
Last NameBean, Ph.D.
First NameHeather
AddressPO Box 874501 Tempe, AZ 85287
Submit Date2020-07-13
Study CommentsStaphylococcus aureus (ATCC 12600) and Stpahylococcus epidermidis (ATCC 12228) grown in four complex media
PublicationsJenkins, C. L., H. D. Bean (2020). Influence of media on the differentiation of Staphylococcus spp. by volatile compounds. Journal of breath research 14, 016007 doi:10.1088/1752-7163/ab3e9d
Raw Data AvailableYes
Raw Data File Type(s).smp
Analysis Type DetailGC-MS
Release Date2020-07-30
Release Version1
Heather Bean Heather Bean
Ph.D. Ph.D. application/zip

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Project ID:PR000978
Project DOI:doi: 10.21228/M88Q44
Project Title:Influence of growth medium on the volatilomes of Pseudomonas spp. and Staphylococcus spp.
Project Type:Untargeted MS
Project Summary:Pseudomonas aeruginosa and Staphylococcus aureus are the predominant opportunistic lung pathogens persons with CF [2017 CF Foundation Annual Report] and are leading causes of respiratory failure and mortality [Malhotra, et al. Clin Microbiol Rev, 2019]. Currently, sputum culture remains the standard of care for lung infection detection, but sputum production is on the decline due to improvements in CF therapies. To fill this diagnostic gap, we are working toward the development of breath tests for lung infections by characterizing the volatile metabolome (or “volatilome”) of P. aeruginosa and S. aureus. In this study, we explored the influence of growth medium on the volatilomes of two strains of P. aeruginosa (PAO1 and PA14) and S. aureus, as well as two other species from the same genera, S. epidermidis and P. chlororaphis. We hypothesized that the volatilomes would be influenced by the growth medium, but that biological differences between these species and strains would dominate the volatilomes and facilitate identification.
Institute:Arizona State University
Department:School of Life Sciences
Laboratory:Heather D. Bean Lab
Last Name:Bean, Ph.D.
First Name:Heather
Address:PO Box 874501 Tempe, AZ 85287
Publications:Jenkins, C. L., H. D. Bean (2020). Influence of media on the differentiation of Staphylococcus spp. by volatile compounds. Journal of breath research 14, 016007 doi:10.1088/1752-7163/ab3e9d