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MGP000431

UniProt Annotations

Entry Information
Gene Namecarbonyl reductase 1
Protein EntryCBR1_HUMAN
UniProt IDP16152
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=P16152-1; Sequence=Displayed; Name=2; IsoId=P16152-2; Sequence=VSP_054796, VSP_054797; Note=No experimental confirmation available.;
Biophysicochemical PropertiesKinetic parameters: KM=30 uM for S-nitrosoglutathione {ECO:0000269|PubMed:17344335, ECO:0000269|PubMed:18826943}; KM=22 uM for menadione {ECO:0000269|PubMed:17344335, ECO:0000269|PubMed:18826943}; KM=309 uM for prostaglandin E2 {ECO:0000269|PubMed:17344335, ECO:0000269|PubMed:18826943}; KM=173 uM for daunorubicin {ECO:0000269|PubMed:17344335, ECO:0000269|PubMed:18826943}; KM=247 uM for NADPH {ECO:0000269|PubMed:17344335, ECO:0000269|PubMed:18826943};
Catalytic Activity(13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprost- 13-enoate + NADP(+) = (13E)-11-alpha-hydroxy-9,15-dioxoprost-13- enoate + NADPH.
Catalytic Activity(5Z,13E)-(15S)-9-alpha,11-alpha,15- trihydroxyprosta-5,13-dienoate + NADP(+) = (5Z,13E)-(15S)-11- alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH.
Catalytic ActivityR-CHOH-R' + NADP(+) = R-CO-R' + NADPH.
Enzyme RegulationInhibited by quercetin, rutenin and its derivatives. {ECO:0000269|PubMed:17344335, ECO:0000269|PubMed:18449627}.
FunctionNADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. {ECO:0000269|PubMed:15799708, ECO:0000269|PubMed:17912391, ECO:0000269|PubMed:18449627, ECO:0000269|PubMed:18826943}.
SimilarityBelongs to the short-chain dehydrogenases/reductases (SDR) family. {ECO:0000305}.
Subcellular LocationCytoplasm.
SubunitMonomer. {ECO:0000269|PubMed:15799708, ECO:0000269|PubMed:17912391, ECO:0000269|PubMed:18826943}.
Web ResourceName=SeattleSNPs; URL="http://pga.gs.washington.edu/data/cbr1/";
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