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MGP000482

UniProt Annotations

Entry Information
Gene Namecell division cycle 20
Protein EntryCDC20_HUMAN
UniProt IDQ12834
SpeciesHuman
Comments
Comment typeDescription
Developmental StageSynthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
FunctionRequired for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. {ECO:0000269|PubMed:9637688, ECO:0000269|PubMed:9734353, ECO:0000269|PubMed:9811605}.
InteractionO88566:Axin2 (xeno); NbExp=2; IntAct=EBI-367462, EBI-7690990; O60566:BUB1B; NbExp=15; IntAct=EBI-367462, EBI-1001438; P30260:CDC27; NbExp=6; IntAct=EBI-367462, EBI-994813; Q13257:MAD2L1; NbExp=16; IntAct=EBI-367462, EBI-78203; Q9UI95:MAD2L2; NbExp=2; IntAct=EBI-367462, EBI-77889; Q9NS23-2:RASSF1; NbExp=2; IntAct=EBI-367462, EBI-438698; Q8IXJ6-2:SIRT2; NbExp=2; IntAct=EBI-367462, EBI-5240785;
PathwayProtein modification; protein ubiquitination.
PtmAcetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C). {ECO:0000269|PubMed:22014574}.
PtmDephosphorylated by CTDP1.
PtmPhosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2. {ECO:0000269|PubMed:10459014, ECO:0000269|PubMed:14657031, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:20034488}.
PtmUbiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex. {ECO:0000269|PubMed:17443180, ECO:0000269|PubMed:18997788, ECO:0000269|PubMed:19098431, ECO:0000269|PubMed:21926987}.
SimilarityBelongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.
SimilarityContains 7 WD repeats. {ECO:0000255|PROSITE- ProRule:PRU00221}.
Subcellular LocationCytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:20034488}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:20034488}.
SubunitFound in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with NEUROD2 and SPATC1 (By similarity). Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2 and SIRT2. Interacts with isoform 1 of NEK2. {ECO:0000250, ECO:0000269|PubMed:11459826, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:17403670, ECO:0000269|PubMed:18997788, ECO:0000269|PubMed:19098431, ECO:0000269|PubMed:19282672, ECO:0000269|PubMed:20034488, ECO:0000269|PubMed:22014574, ECO:0000269|PubMed:9637688, ECO:0000269|PubMed:9734353, ECO:0000269|PubMed:9811605}.
Web ResourceName=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/CDC20ID40003ch1p34.html";
Web ResourceName=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/cdc20/";
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