MGP Database

New search | Record Overview | Ontology/Pathway Information | Domain Information | UniProt Annotations | Related Proteins
MGP001155

UniProt Annotations

Entry Information
Gene Nameglyceraldehyde-3-phosphate dehydrogenase
Protein EntryG3P_HUMAN
UniProt IDP04406
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=P04406-1; Sequence=Displayed; Name=2; IsoId=P04406-2; Sequence=VSP_047289; Note=No experimental confirmation available. Gene prediction based on EST data.;
Catalytic ActivityD-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH. {ECO:0000255|PROSITE-ProRule:PRU10009, ECO:0000269|PubMed:3170585}.
FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. {ECO:0000250, ECO:0000269|PubMed:11724794, ECO:0000269|PubMed:23071094, ECO:0000269|PubMed:3170585}.
InteractionSelf; NbExp=2; IntAct=EBI-354056, EBI-354056; Q60823:Akt2 (xeno); NbExp=2; IntAct=EBI-354056, EBI-400263; P00533:EGFR; NbExp=6; IntAct=EBI-354056, EBI-297353; P12004:PCNA; NbExp=3; IntAct=EBI-354056, EBI-358311; P00558:PGK1; NbExp=2; IntAct=EBI-354056, EBI-709599; P48147:PREP; NbExp=5; IntAct=EBI-354056, EBI-1049962; P15927:RPA2; NbExp=2; IntAct=EBI-354056, EBI-621404;
PathwayCarbohydrate degradation; glycolysis; pyruvate from D- glyceraldehyde 3-phosphate: step 1/5.
PtmISGylated. {ECO:0000305|PubMed:16815975}.
PtmS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. {ECO:0000250}.
PtmSulfhydration at Cys-152 increases catalytic activity. {ECO:0000250}.
SimilarityBelongs to the glyceraldehyde-3-phosphate dehydrogenase family. {ECO:0000305}.
Subcellular LocationCytoplasm, cytosol {ECO:0000269|PubMed:12829261}. Nucleus {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:12829261}. Membrane {ECO:0000269|PubMed:12829261}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. {ECO:0000250}.
SubunitHomotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules (By similarity). Interacts with EIF1AD, USP25, PRKCI and WARS. Component of the GAIT complex. Interacts with FKBP6; leading to inhibit GAPDH catalytic activity. {ECO:0000250, ECO:0000269|PubMed:11724794, ECO:0000269|PubMed:15479637, ECO:0000269|PubMed:15628863, ECO:0000269|PubMed:16239728, ECO:0000269|PubMed:16501887, ECO:0000269|PubMed:16510976, ECO:0000269|PubMed:19001379, ECO:0000269|PubMed:20644585}.
Web ResourceName=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/gapd/";
Web ResourceName=Wikipedia; Note=Glyceraldehyde 3-phosphate dehydrogenase entry; URL="http://en.wikipedia.org/wiki/Glyceraldehyde_3-phosphate_dehydrogenase";
  logo