MGP Database

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MGP001466

UniProt Annotations

Entry Information
Gene Name3-hydroxy-3-methylglutaryl-CoA reductase
Protein EntryHMDH_HUMAN
UniProt IDP04035
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=P04035-1; Sequence=Displayed; Name=2; IsoId=P04035-2; Sequence=VSP_002207; Note=No experimental confirmation available.; Name=3; Synonyms=HMGCR-1b; IsoId=P04035-3; Sequence=VSP_046492; Note=Most highly expressed transcript in skin, esophagus, and uterine cervix.;
Catalytic Activity(R)-mevalonate + CoA + 2 NADP(+) = (S)-3- hydroxy-3-methylglutaryl-CoA + 2 NADPH. {ECO:0000255|PROSITE- ProRule:PRU10003}.
Enzyme RegulationRegulated by a negative feedback mechanism through sterols and non-sterol metabolites derived from mevalonate. Inhibited by statins, a class of hypolipidemic agents used as pharmaceuticals to lower cholesterol levels in individuals at risk from cardiovascular disease due to hypercholesterolemia. Inhibition of HMGCR in the liver stimulates the LDL-receptors, which results in an increased clearance of LDL from the bloodstream and a decrease in blood cholesterol levels. The first results can be seen after one week of statin use and the effect is maximal after four to six weeks. {ECO:0000269|PubMed:6995544}.
FunctionTransmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.
InteractionQ9Y5Z9:UBIAD1; NbExp=3; IntAct=EBI-465513, EBI-2819725; Q9Y5Z9-1:UBIAD1; NbExp=5; IntAct=EBI-465513, EBI-6621921;
PathwayMetabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
PtmN-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner. {ECO:0000269|PubMed:19458199}.
PtmUndergoes sterol-mediated ubiquitination and ER-association degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1. This INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein. {ECO:0000269|PubMed:12535518, ECO:0000269|PubMed:19458199, ECO:0000269|PubMed:21778231}.
Sequence CautionSequence=BAH12375.1; Type=Frameshift; Positions=122; Evidence={ECO:0000305};
SimilarityBelongs to the HMG-CoA reductase family. {ECO:0000305}.
SimilarityContains 1 SSD (sterol-sensing) domain. {ECO:0000255|PROSITE-ProRule:PRU00199}.
Subcellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein.
SubunitHomodimer. Interacts (via its SSD) with INSIG1; the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway. Interacts with UBIAD1. {ECO:0000269|PubMed:10698924, ECO:0000269|PubMed:12535518, ECO:0000269|PubMed:18540668, ECO:0000269|PubMed:19458199, ECO:0000269|PubMed:23169578}.
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