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MGP001842

UniProt Annotations

Entry Information
Gene Namecytochrome P450, family 4, subfamily F, polypeptide 3
Protein EntryCP4F3_HUMAN
UniProt IDQ08477
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative promoter usage, Alternative splicing; Named isoforms=2; Name=CYP4F3A; IsoId=Q08477-1; Sequence=Displayed; Name=CYP4F3B; IsoId=Q08477-2; Sequence=VSP_047193;
Catalytic Activity(5Z,8Z,11Z,14Z)-icosatetraenoate + NADPH + O(2) = (5Z,8Z,11Z,14Z)-20-hydroxyicosa-5,8,11,14-tetraenoate + NADP(+) + H(2)O. {ECO:0000269|PubMed:11461919, ECO:0000269|PubMed:16820285}.
Catalytic Activity(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa- 6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)- 5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O. {ECO:0000269|PubMed:8486631}.
Catalytic ActivityDocosahexaenoate + NADPH + O(2) = 22- hydroxydocosahexaenoate + NADP(+) + H(2)O. {ECO:0000269|PubMed:16820285}.
CofactorName=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250|UniProtKB:P51869};
Enzyme RegulationInhibited by carbon monoxide (CO).
FunctionIsoform CYP4F3A: Catalyzes the omega-hydroxylation of leukotriene-B(4), a potent chemoattractant for polymorphonuclear leukocytes, it has low activity for arachidonic acid. {ECO:0000269|PubMed:11461919, ECO:0000269|PubMed:8486631}.
FunctionIsoform CYP4F3B: Shows arachidonic acid omega- hydroxylase activity by mediating conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE) (PubMed:11461919, PubMed:16820285). Has a 30-fold higher Km for leukotriene-B(4) compared with CYP4F3A (PubMed:11461919). Also converts eicosapentaenoate (EPA) to 20-hydroxyeicosapentaenoate (20-OH-EPA) (PubMed:16820285). Acts as a polyunsaturated omega-3 fatty acids hydroxylase: mediates conversion of docosahexaenoate (DHA) to 22- hydroxydocosahexaenoate (PubMed:16820285). {ECO:0000269|PubMed:11461919, ECO:0000269|PubMed:16820285, ECO:0000269|PubMed:18577768}.
PathwayLipid metabolism; leukotriene B4 degradation.
SimilarityBelongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Microsome membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
Tissue SpecificityIsoform CYP4F3A is expressed in the polymorphonuclear leukocytes as well as leukocytes and bone marrow. Isoform CYP4F3B is selectively expressed in liver and kidney and is also the predominant CYP4F isoform in trachea and tissues of the gastrointestinal tract. {ECO:0000269|PubMed:11461919}.
Web ResourceName=SeattleSNPs; URL="http://pga.gs.washington.edu/data/cyp4f3/";
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