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MGP001935

UniProt Annotations

Entry Information
Gene Namematrix metallopeptidase 1 (interstitial collagenase)
Protein EntryMMP1_HUMAN
UniProt IDP03956
SpeciesHuman
Comments
Comment typeDescription
Catalytic ActivityCleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N- terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.
CofactorName=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 4 Ca(2+) ions per subunit.;
CofactorName=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per subunit.;
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
DomainThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
Enzyme RegulationCan be activated without removal of the activation peptide.
FunctionCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. {ECO:0000269|PubMed:1645757}.
PtmTyrosine phosphorylated in platelets by PKDCC/VLK. {ECO:0000269|PubMed:25171405}.
PtmUndergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. {ECO:0000269|PubMed:10092871}.
SimilarityBelongs to the peptidase M10A family. {ECO:0000305}.
SimilarityContains 4 hemopexin repeats. {ECO:0000305}.
Subcellular LocationSecreted, extracellular space, extracellular matrix {ECO:0000305|PubMed:2167156}.
SubunitInteracts with HIV-1 Tat. {ECO:0000269|PubMed:16807369}.
Web ResourceName=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/mmp1/";
Web ResourceName=Wikipedia; Note=Collagenase entry; URL="http://en.wikipedia.org/wiki/Collagenase";
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