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MGP002292

UniProt Annotations

Entry Information

Gene Name	phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit delta
Protein Entry	PK3CD_HUMAN
UniProt ID	O00329
Species	Human

Comments

Comment type	Description
Alternative Products	Event=Alternative splicing; Named isoforms=2; Name=1; Synonyms=p110-delta; IsoId=O00329-1; Sequence=Displayed; Name=2; Synonyms=p37-delta; IsoId=O00329-2; Sequence=VSP_044409, VSP_044410;
Catalytic Activity	ATP + 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5- trisphosphate.
Disease	Activated PI3K-delta syndrome (APDS) [MIM:615513]: A disorder characterized by recurrent respiratory infections, progressive airway damage, lymphopenia, increased circulating transitional B cells, increased immunoglobulin M, reduced immunoglobulin G2 levels in serum, and impaired vaccine responses. {ECO:0000269\|PubMed:24136356}. Note=The disease is caused by mutations affecting the gene represented in this entry.
Enzyme Regulation	Activated by growth factors and cytokine receptors through a tyrosine-kinase-dependent mechanism. Activated by RAS. IC87114 inhibits lipid kinase activity and is selective in cells at doses up to 5-10 uM. IC87114 blocks T-cell receptor signaling in naive and memory T-cells and reduces cytokine production by memory T-cells. {ECO:0000269\|PubMed:20081091}.
Function	Phosphoinositide-3-kinase (PI3K) that phosphorylates PftdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Have a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Have important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function. Isoform 2 may be involved in stabilizing total RAS levels, resulting in increased ERK phosphorylation and increased PI3K activity. {ECO:0000269\|PubMed:20081091, ECO:0000269\|PubMed:22020336}.
Interaction	P01112:HRAS; NbExp=2; IntAct=EBI-718309, EBI-350145;
Miscellaneous	IC87114 inhibitor reduces passive cutaneous anaphylaxis, attenuates allergic airway inflammation and hyperresponsiveness and allergen induced rhinitis response. Inhibitors may have therapeutic potential for the treatment of immune system-mediated diseases such as auto-immune diseases, inflammation and allergy (PubMed:20940048; PubMed:17290298).
Pathway	Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Ptm	Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity. {ECO:0000269\|PubMed:10064595, ECO:0000269\|PubMed:19369195}.
Similarity	Belongs to the PI3/PI4-kinase family. {ECO:0000255\|PROSITE-ProRule:PRU00877, ECO:0000255\|PROSITE- ProRule:PRU00879, ECO:0000255\|PROSITE-ProRule:PRU00880}.
Similarity	Contains 1 C2 PI3K-type domain. {ECO:0000255\|PROSITE- ProRule:PRU00880}.
Similarity	Contains 1 PI3K-ABD domain. {ECO:0000255\|PROSITE- ProRule:PRU00877}.
Similarity	Contains 1 PI3K/PI4K domain. {ECO:0000255\|PROSITE- ProRule:PRU00269}.
Similarity	Contains 1 PI3K-RBD domain. {ECO:0000255\|PROSITE- ProRule:PRU00879}.
Similarity	Contains 1 PIK helical domain. {ECO:0000255\|PROSITE- ProRule:PRU00878}.
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:22020336}.
Subunit	Heterodimer of a catalytic subunit PIK3CD and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with ERAS (By similarity). Interacts with HRAS. {ECO:0000250, ECO:0000269\|PubMed:22020336}.
Tissue Specificity	Isoform 2 is expressed in normal thymus, lung and spleen tissues, and is detected at low levels in normal lysates from colon and ovarian biopsies, at elevated levels in lysates from colorectal tumors and is abundantly expressed in some ovarian tumors (at protein level). Both isoform 1 and isoform 2 are widely expressed. Isoform 1 is expressed predominantly in leukocytes. {ECO:0000269\|PubMed:22020336}.
Web Resource	Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/PIK3CDID46261ch1p36.html";