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MGP002378

UniProt Annotations

Entry Information
Gene Namemediator complex subunit 1
Protein EntryMED1_HUMAN
UniProt IDQ15648
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q15648-1; Sequence=Displayed; Name=2; IsoId=Q15648-3; Sequence=VSP_027906, VSP_027907;
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:10406464, ECO:0000269|PubMed:11867769, ECO:0000269|PubMed:12037571, ECO:0000269|PubMed:12218053, ECO:0000269|PubMed:12556447, ECO:0000269|PubMed:14636573, ECO:0000269|PubMed:15340084, ECO:0000269|PubMed:15471764, ECO:0000269|PubMed:15989967, ECO:0000269|PubMed:16574658, ECO:0000269|PubMed:9653119}.
InteractionO43513:MED7; NbExp=4; IntAct=EBI-394459, EBI-394632; P10276:RARA; NbExp=2; IntAct=EBI-394459, EBI-413374; P10827:THRA; NbExp=5; IntAct=EBI-394459, EBI-286285;
PtmPhosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. {ECO:0000269|PubMed:16314496, ECO:0000269|PubMed:16964243, ECO:0000269|PubMed:18669648, ECO:0000269|PubMed:18691976, ECO:0000269|PubMed:19369195, ECO:0000269|PubMed:19690332, ECO:0000269|PubMed:20068231, ECO:0000269|PubMed:21406692}.
Sequence CautionSequence=AAC39854.1; Type=Frameshift; Positions=543, 545; Evidence={ECO:0000305}; Sequence=AAH06517.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305}; Sequence=CAA73867.1; Type=Frameshift; Positions=4; Evidence={ECO:0000305};
SimilarityBelongs to the Mediator complex subunit 1 family. {ECO:0000305}.
Subcellular LocationNucleus {ECO:0000269|PubMed:15471764, ECO:0000269|PubMed:16314496, ECO:0000269|PubMed:16574658}. Note=A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
SubunitComponent of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. This subunit specifically interacts with a number of nuclear receptors in a ligand-dependent fashion including AR, ESR1, ESR2, PPARA, PPARG, RORA, RXRA, RXRG, THRA, THRB and VDR. Interacts with CTNNB1, GABPA, GLI3, PPARGC1A and TP53. Interacts with GATA1 and YWHAH. Interacts with CLOCK; this interaction requires the presence of THRAP3 (By similarity). {ECO:0000250|UniProtKB:Q925J9, ECO:0000269|PubMed:10024883, ECO:0000269|PubMed:10235266, ECO:0000269|PubMed:10235267, ECO:0000269|PubMed:10406464, ECO:0000269|PubMed:10478845, ECO:0000269|PubMed:10733574, ECO:0000269|PubMed:10770935, ECO:0000269|PubMed:11303023, ECO:0000269|PubMed:11867769, ECO:0000269|PubMed:12037571, ECO:0000269|PubMed:12218053, ECO:0000269|PubMed:12556447, ECO:0000269|PubMed:14636573, ECO:0000269|PubMed:15175163, ECO:0000269|PubMed:15340084, ECO:0000269|PubMed:15471764, ECO:0000269|PubMed:15989967, ECO:0000269|PubMed:16574658, ECO:0000269|PubMed:17000779, ECO:0000269|PubMed:19786558, ECO:0000269|PubMed:9444950, ECO:0000269|PubMed:9653119}.
Tissue SpecificityUbiquitously expressed. {ECO:0000269|PubMed:9444950, ECO:0000269|PubMed:9653119}.
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