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MGP002399

UniProt Annotations

Entry Information
Gene Nameprotein phosphatase 2, regulatory subunit B', gamma
Protein Entry2A5G_HUMAN
UniProt IDQ13362
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=5; Name=Gamma-3; IsoId=Q13362-1; Sequence=Displayed; Name=Gamma-1; IsoId=Q13362-2; Sequence=VSP_005112; Name=Gamma-2; IsoId=Q13362-3; Sequence=VSP_005113; Name=4; IsoId=Q13362-4; Sequence=VSP_043645, VSP_005113; Note=No experimental confirmation available.; Name=5; IsoId=Q13362-5; Sequence=VSP_046768; Note=Ref.3 (BAG63760) sequence is in conflict in position: 3:N->K. No experimental confirmation available. {ECO:0000305};
FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage- induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation. {ECO:0000269|PubMed:16456541, ECO:0000269|PubMed:17245430}.
InductionUp-regulated upon DNA damage. {ECO:0000269|PubMed:17245430}.
InteractionO96017:CHEK2; NbExp=4; IntAct=EBI-1266176, EBI-1180783; P46695:IER3; NbExp=2; IntAct=EBI-1266156, EBI-1748945; Q00987:MDM2; NbExp=5; IntAct=EBI-1266156, EBI-389668; P67775:PPP2CA; NbExp=7; IntAct=EBI-1266156, EBI-712311; P30153:PPP2R1A; NbExp=6; IntAct=EBI-1266156, EBI-302388; P30154:PPP2R1B; NbExp=2; IntAct=EBI-1266156, EBI-357094; P04637:TP53; NbExp=4; IntAct=EBI-1266156, EBI-366083;
PtmIsoform Gamma-3 is phosphorylated on serine residues. Isoform Gamma-1 phosphorylation by ERK2 is IER3-dependent and inhibits ERK dephosphorylation by PP2A-PPP2R5C. {ECO:0000269|PubMed:16456541, ECO:0000269|PubMed:8703017}.
Sequence CautionSequence=AAC50387.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAG63760.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
SimilarityBelongs to the phosphatase 2A regulatory subunit B56 family. {ECO:0000305}.
Subcellular LocationNucleus. Chromosome, centromere.
SubunitPP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with PPP2CA AND PPP2R1A; the interaction is direct. Interacts with SGOL1; the interaction is direct. Isoform 1 and isoform 2 interact with TP53 (phosphorylated at Ser-15 by ATM); increased upon DNA damage it drives PP2A-mediated dephosphorylation of TP53 at Thr- 55. Interacts with IER3 and/or ERK kinases; regulates ERK dephosphorylation. {ECO:0000269|PubMed:16456541, ECO:0000269|PubMed:16541025, ECO:0000269|PubMed:16580887, ECO:0000269|PubMed:17086192, ECO:0000269|PubMed:17174897, ECO:0000269|PubMed:17245430, ECO:0000269|PubMed:17967874, ECO:0000269|PubMed:19716788}.
Tissue SpecificityHighest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.
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