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MGP002542

UniProt Annotations

Entry Information

Gene Name	paxillin
Protein Entry	PAXI_HUMAN
UniProt ID	P49023
Species	Human

Comments

Comment type	Description
Alternative Products	Event=Alternative splicing; Named isoforms=4; Name=Beta; IsoId=P49023-1; Sequence=Displayed; Name=Alpha; IsoId=P49023-2; Sequence=VSP_003114; Name=Gamma; IsoId=P49023-3; Sequence=VSP_003115; Name=4; IsoId=P49023-4; Sequence=VSP_040483, VSP_003114;
Function	Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Interaction	P41240:CSK; NbExp=2; IntAct=EBI-702209, EBI-1380630; P35222:CTNNB1; NbExp=4; IntAct=EBI-702209, EBI-491549; Q13480:GAB1; NbExp=2; IntAct=EBI-702209, EBI-517684; Q14161:GIT2; NbExp=2; IntAct=EBI-702209, EBI-1046878; P13612:ITGA4; NbExp=3; IntAct=EBI-702209, EBI-703044; P05556-1:ITGB1; NbExp=2; IntAct=EBI-702209, EBI-6082935; P27986:PIK3R1; NbExp=2; IntAct=EBI-702209, EBI-79464; Q05397:PTK2; NbExp=15; IntAct=EBI-702209, EBI-702142; Q06124:PTPN11; NbExp=3; IntAct=EBI-702209, EBI-297779; Q09463:rnf-5 (xeno); NbExp=2; IntAct=EBI-702209, EBI-963421; Q99942:RNF5; NbExp=6; IntAct=EBI-702209, EBI-348482; Q62417-2:Sorbs1 (xeno); NbExp=8; IntAct=EBI-702209, EBI-7072893; P05480:Src (xeno); NbExp=2; IntAct=EBI-702209, EBI-298680;
Ptm	Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. {ECO:0000250, ECO:0000269\|PubMed:11774284, ECO:0000269\|PubMed:15572663, ECO:0000269\|PubMed:16964243, ECO:0000269\|PubMed:18042622, ECO:0000269\|PubMed:18220336, ECO:0000269\|PubMed:18669648, ECO:0000269\|PubMed:19690332, ECO:0000269\|PubMed:20068231, ECO:0000269\|PubMed:21406692}.
Similarity	Belongs to the paxillin family. {ECO:0000305}.
Similarity	Contains 4 LIM zinc-binding domains. {ECO:0000255\|PROSITE-ProRule:PRU00125}.
Subcellular Location	Cytoplasm, cytoskeleton {ECO:0000269\|PubMed:20489202}. Cell junction, focal adhesion {ECO:0000269\|PubMed:20489202}. Cytoplasm, cell cortex {ECO:0000250}. Note=Colocalizes with integrins at the cell periphery. {ECO:0000250}.
Subunit	Binds in vitro to vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of V- CRK. Isoform beta binds to PTK2/FAK1 but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to PTK2/FAK1. Interacts with GIT1, NUDT16L1/SDOS and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Interacts with PTK2/FAK1 and PTK2B/PYK2. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5 and PDCD10. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with PTK6. Interacts with SORBS1, PARVA and PARVB. {ECO:0000269\|PubMed:10604475, ECO:0000269\|PubMed:10749932, ECO:0000269\|PubMed:10938112, ECO:0000269\|PubMed:12861019, ECO:0000269\|PubMed:14527389, ECO:0000269\|PubMed:15572663, ECO:0000269\|PubMed:17297458, ECO:0000269\|PubMed:17462669, ECO:0000269\|PubMed:18508764, ECO:0000269\|PubMed:18940607, ECO:0000269\|PubMed:19358827, ECO:0000269\|PubMed:20037584, ECO:0000269\|PubMed:20489202, ECO:0000269\|PubMed:22869380}.
Web Resource	Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/PXNID41953ch12q24.html";