MGP Database

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MGP002562

UniProt Annotations

Entry Information
Gene NameRAD9 homolog A (S. pombe)
Protein EntryRAD9A_HUMAN
UniProt IDQ99638
SpeciesHuman
Comments
Comment typeDescription
Catalytic ActivityExonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates.
FunctionComponent of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex. {ECO:0000269|PubMed:10713044, ECO:0000269|PubMed:21659603}.
InteractionO60921:HUS1; NbExp=5; IntAct=EBI-2606224, EBI-1056174; O60671:RAD1; NbExp=2; IntAct=EBI-2606224, EBI-721835;
PtmConstitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex. {ECO:0000269|PubMed:11971963, ECO:0000269|PubMed:12628935, ECO:0000269|PubMed:12709442, ECO:0000269|PubMed:17081983, ECO:0000269|PubMed:18669648, ECO:0000269|PubMed:19690332, ECO:0000269|PubMed:20068231, ECO:0000269|PubMed:21406692}.
SimilarityBelongs to the rad9 family. {ECO:0000305}.
Subcellular LocationNucleus {ECO:0000269|PubMed:12628935}.
SubunitComponent of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1. {ECO:0000269|PubMed:10359610, ECO:0000269|PubMed:10777662, ECO:0000269|PubMed:10846170, ECO:0000269|PubMed:10884395, ECO:0000269|PubMed:11573955, ECO:0000269|PubMed:11971963, ECO:0000269|PubMed:12628935, ECO:0000269|PubMed:14500360, ECO:0000269|PubMed:15314187, ECO:0000269|PubMed:15556996, ECO:0000269|PubMed:15871698, ECO:0000269|PubMed:15897895, ECO:0000269|PubMed:16216273, ECO:0000269|PubMed:21659603}.
Web ResourceName=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/RAD9AID42031ch11q13.html";
Web ResourceName=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/rad9a/";
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