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MGP003173

UniProt Annotations

Entry Information
Gene Nametopoisomerase (DNA) II beta 180kDa
Protein EntryTOP2B_HUMAN
UniProt IDQ02880
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=Beta-2; IsoId=Q02880-1; Sequence=Displayed; Name=Beta-1; IsoId=Q02880-2; Sequence=VSP_006532;
Catalytic ActivityATP-dependent breakage, passage and rejoining of double-stranded DNA. {ECO:0000255|PROSITE-ProRule:PRU00995, ECO:0000269|PubMed:10684600}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|PROSITE-ProRule:PRU00995, ECO:0000269|PubMed:10684600, ECO:0000269|PubMed:21778401}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|PROSITE-ProRule:PRU00995, ECO:0000269|PubMed:10684600, ECO:0000269|PubMed:21778401}; Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000255|PROSITE-ProRule:PRU00995, ECO:0000269|PubMed:10684600, ECO:0000269|PubMed:21778401}; Note=Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+). {ECO:0000255|PROSITE-ProRule:PRU00995, ECO:0000269|PubMed:10684600, ECO:0000269|PubMed:21778401};
FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. {ECO:0000269|PubMed:10684600, ECO:0000269|PubMed:12837248}.
MiscellaneousEukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.
SimilarityBelongs to the type II topoisomerase family. {ECO:0000305}.
SimilarityContains 1 Toprim domain. {ECO:0000255|PROSITE- ProRule:PRU00995}.
Subcellular LocationCytoplasm. Nucleus, nucleolus.
SubunitHomodimer. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. {ECO:0000269|PubMed:12837248, ECO:0000269|PubMed:21778401}.
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