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MGP003233

UniProt Annotations

Entry Information
Gene NameU2 small nuclear RNA auxiliary factor 1
Protein EntryU2AF1_HUMAN
UniProt IDQ01081
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=4; Name=1; Synonyms=U2AF35a; IsoId=Q01081-1; Sequence=Displayed; Name=2; Synonyms=U2AF35b; IsoId=Q01081-2; Sequence=VSP_042665; Note=Interacts with U2AF2 and stimulates U2AF splicing activity in vitro. Less efficient than isoform 1.; Name=3; Synonyms=U2AF35c; IsoId=Q01081-3; Sequence=VSP_042665, VSP_042666, VSP_042667; Note=Produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.; Name=4; IsoId=Q01081-4; Sequence=VSP_042664;
DomainThe C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.
FunctionPlays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. {ECO:0000269|PubMed:8647433}.
InteractionQ86X95:CIR1; NbExp=4; IntAct=EBI-632461, EBI-627102; Q6NYC1:JMJD6; NbExp=2; IntAct=EBI-632461, EBI-8464037; Q8N5F7:NKAP; NbExp=2; IntAct=EBI-632461, EBI-721539; Q13435:SF3B2; NbExp=2; IntAct=EBI-632461, EBI-749111; P78362:SRPK2; NbExp=4; IntAct=EBI-632461, EBI-593303; O43463:SUV39H1; NbExp=2; IntAct=EBI-632461, EBI-349968; P26368:U2AF2; NbExp=8; IntAct=EBI-632461, EBI-742339;
SimilarityBelongs to the splicing factor SR family. {ECO:0000305}.
SimilarityContains 1 RRM (RNA recognition motif) domain. {ECO:0000255|PROSITE-ProRule:PRU00176}.
SimilarityContains 2 C3H1-type zinc fingers. {ECO:0000255|PROSITE-ProRule:PRU00723}.
Subcellular LocationNucleus {ECO:0000269|PubMed:15096518}. Nucleus speckle {ECO:0000269|PubMed:15096518}.
SubunitInteracts (via RS domain) with PHF5A (via N-terminus) (By similarity). Identified in the spliceosome C complex. Heterodimer with U2AF2. Interacts with ZRANB2. {ECO:0000250, ECO:0000269|PubMed:11448987, ECO:0000269|PubMed:11551507, ECO:0000269|PubMed:11991638}.
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