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MGP003382

UniProt Annotations

Entry Information
Gene Nametripartite motif containing 25
Protein EntryTRI25_HUMAN
UniProt IDQ14258
SpeciesHuman
Comments
Comment typeDescription
Catalytic ActivityATP + [ISG15] + [protein]-lysine = AMP + diphosphate + [protein]-N-ISGyllysine.
Catalytic ActivityATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
DomainThe C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
DomainThe RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
FunctionFunctions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. {ECO:0000269|PubMed:16352599, ECO:0000269|PubMed:17069755, ECO:0000269|PubMed:17392790}.
InductionBy interferons. {ECO:0000269|PubMed:16352599, ECO:0000269|PubMed:17069755}.
InteractionF1BA49:- (xeno); NbExp=5; IntAct=EBI-2341129, EBI-9687469; O95786:DDX58; NbExp=4; IntAct=EBI-2341129, EBI-995350; P03496:NS (xeno); NbExp=3; IntAct=EBI-2341129, EBI-2547442;
PathwayProtein modification; protein ubiquitination.
PtmAuto-ISGylated. {ECO:0000269|PubMed:17222803}.
SimilarityContains 1 B30.2/SPRY domain. {ECO:0000255|PROSITE- ProRule:PRU00548}.
SimilarityContains 1 RING-type zinc finger. {ECO:0000255|PROSITE-ProRule:PRU00175}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:17392790}. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies.
SubunitInteracts (via SPRY domain) with DDX58 (via CARD domain). Interacts (via coiled coil) with influenza A virus NS1 protein; this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD ubiquitination, thereby suppressing DDX58 signal transduction. {ECO:0000269|PubMed:17392790, ECO:0000269|PubMed:19454348}.
Tissue SpecificityUbiquitous. {ECO:0000269|PubMed:15130519}.
Web ResourceName=Wikipedia; Note=TRIM25 entry; URL="http://en.wikipedia.org/wiki/TRIM25";
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