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MGP003623

UniProt Annotations

Entry Information
Gene Namehydroxysteroid (17-beta) dehydrogenase 6
Protein EntryH17B6_HUMAN
UniProt IDO14756
SpeciesHuman
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=0.19 uM for NAD {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; KM=0.18 uM for NADH {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; KM=54 uM for NADPH {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; KM=940 uM for NADP {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; KM=3.2 uM for all-trans-retinol {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; KM=0.24 uM for allopregnanolone {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; KM=0.13 uM for 3-alpha-androstanediol {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; KM=0.23 uM for androsterone {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; KM=0.13 uM for dehydroepiandrosterone {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; Vmax=1.2 nmol/min/mg enzyme with all-trans-retinol {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; Vmax=14.7 nmol/min/mg enzyme with allopregnanolone {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; Vmax=16.5 nmol/min/mg enzyme with 3-alpha-androstanediol {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; Vmax=35 nmol/min/mg enzyme with androsterone {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; Vmax=0.90 nmol/min/mg enzyme with dehydroepiandrosterone {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992}; Note=The kinetic parameters were determined using microsomes from transfected cells.;
Catalytic Activity17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H.
Catalytic ActivityAll-trans-retinol-[cellular-retinol-binding- protein] + NAD(+) = all-trans-retinal-[cellular-retinol-binding- protein] + NADH.
Catalytic ActivityTestosterone + NAD(+) = androstenedione + NADH.
FunctionNAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3- alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha- androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids. {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:11360992, ECO:0000269|PubMed:11513953}.
Sequence CautionSequence=AAB88252.1; Type=Frameshift; Positions=158, 174; Evidence={ECO:0000305};
SimilarityBelongs to the short-chain dehydrogenases/reductases (SDR) family. {ECO:0000305}.
Subcellular LocationMicrosome membrane {ECO:0000269|PubMed:11360992}; Peripheral membrane protein {ECO:0000269|PubMed:11360992}; Lumenal side {ECO:0000269|PubMed:11360992}. Early endosome membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Lumenal side {ECO:0000305}.
Tissue SpecificityDetected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain. {ECO:0000269|PubMed:10896656, ECO:0000269|PubMed:9188497}.
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