MGP Database

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MGP003637

UniProt Annotations

Entry Information
Gene Nameeukaryotic translation initiation factor 3, subunit B
Protein EntryEIF3B_HUMAN
UniProt IDP55884
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=P55884-1; Sequence=Displayed; Name=2; IsoId=P55884-2; Sequence=VSP_017274;
DomainThe RRM domain mediates interaction with EIF3J.
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. {ECO:0000269|PubMed:9388245}.
InteractionO00571:DDX3X; NbExp=5; IntAct=EBI-366696, EBI-353779; Q14152:EIF3A; NbExp=6; IntAct=EBI-366696, EBI-366617; O00303:EIF3F; NbExp=3; IntAct=EBI-366696, EBI-711990; O75821:EIF3G; NbExp=4; IntAct=EBI-366696, EBI-366632; Q13347:EIF3I; NbExp=4; IntAct=EBI-366696, EBI-354047; O75822:EIF3J; NbExp=5; IntAct=EBI-366696, EBI-366647;
Mass SpectrometryMass=92561; Mass_error=43.5; Method=MALDI; Range=1-814; Evidence={ECO:0000269|PubMed:18599441};
Mass SpectrometryMass=93093.7; Method=Unknown; Range=1-814; Evidence={ECO:0000269|PubMed:17322308};
PtmPhosphorylated. Phosphorylation is enhanced upon serum stimulation. {ECO:0000255|HAMAP-Rule:MF_03001, ECO:0000269|PubMed:17081983, ECO:0000269|PubMed:17322308, ECO:0000269|PubMed:18669648, ECO:0000269|PubMed:19690332, ECO:0000269|PubMed:20068231, ECO:0000269|PubMed:21406692}.
SimilarityBelongs to the eIF-3 subunit B family. {ECO:0000255|HAMAP-Rule:MF_03001}.
SimilarityContains 1 RRM (RNA recognition motif) domain. {ECO:0000255|HAMAP-Rule:MF_03001}.
SimilarityContains 5 WD repeats. {ECO:0000255|HAMAP- Rule:MF_03001}.
Subcellular LocationCytoplasm {ECO:0000255|HAMAP-Rule:MF_03001}.
SubunitComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2 and HNRPD. {ECO:0000255|HAMAP-Rule:MF_03001, ECO:0000269|PubMed:11169732, ECO:0000269|PubMed:11457827, ECO:0000269|PubMed:14519125, ECO:0000269|PubMed:14688252, ECO:0000269|PubMed:16286006, ECO:0000269|PubMed:16766523, ECO:0000269|PubMed:17190833, ECO:0000269|PubMed:17322308, ECO:0000269|PubMed:17468741, ECO:0000269|PubMed:18599441, ECO:0000269|PubMed:24423872}.
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