MGP Database

New search | Record Overview | Ontology/Pathway Information | Domain Information | UniProt Annotations | Related Proteins
MGP003840

UniProt Annotations

Entry Information
Gene Nameribosomal protein S6 kinase, 90kDa, polypeptide 5
Protein EntryKS6A5_HUMAN
UniProt IDO75582
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=O75582-1; Sequence=Displayed; Name=2; IsoId=O75582-2; Sequence=VSP_041837, VSP_041838; Name=3; IsoId=O75582-3; Sequence=VSP_057410; Note=No experimental confirmation available.;
Catalytic ActivityATP + a protein = ADP + a phosphoprotein. {ECO:0000269|PubMed:12628924, ECO:0000269|PubMed:9687510, ECO:0000269|PubMed:9873047}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:12628924, ECO:0000269|PubMed:9687510, ECO:0000269|PubMed:9873047};
Enzyme RegulationActivated by phosphorylation at Ser-360, Thr- 581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha, and by further autophosphorylation of Ser-212, Ser-376 and Ser-381 by the activated C-terminal kinase domain. The active N-terminal kinase domain finally phosphorylates downstream substrates, as well as Ser-750, Ser-752 and Ser-758 in its own C-terminal region. {ECO:0000269|PubMed:15568999, ECO:0000269|PubMed:9687510}.
FunctionSerine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto- oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro- inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti- inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury. {ECO:0000269|PubMed:11909979, ECO:0000269|PubMed:12569367, ECO:0000269|PubMed:12628924, ECO:0000269|PubMed:12763138, ECO:0000269|PubMed:12773393, ECO:0000269|PubMed:15010469, ECO:0000269|PubMed:18511904, ECO:0000269|PubMed:9687510, ECO:0000269|PubMed:9873047}.
InteractionQ04206:RELA; NbExp=2; IntAct=EBI-73869, EBI-73886; Q9NYL2:ZAK; NbExp=4; IntAct=EBI-73869, EBI-602273;
MiscellaneousEnzyme activity requires the presence of both kinase domains. {ECO:0000269|PubMed:9687510}.
PtmSer-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C- terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-360, Thr-581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-750, Ser-752 and Ser-758 by the N-terminal kinase domain. {ECO:0000269|PubMed:15568999, ECO:0000269|PubMed:17117922, ECO:0000269|PubMed:19690332, ECO:0000269|PubMed:20068231}.
PtmUbiquitinated. {ECO:0000269|PubMed:20596523}.
Sequence CautionSequence=AAC69577.1; Type=Frameshift; Positions=Several; Evidence={ECO:0000305};
SimilarityBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. {ECO:0000305}.
SimilarityContains 1 AGC-kinase C-terminal domain. {ECO:0000305}.
SimilarityContains 2 protein kinase domains. {ECO:0000255|PROSITE-ProRule:PRU00159}.
Subcellular LocationNucleus. Cytoplasm. Note=Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.
SubunitForms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA. Interacts with CREBBP and EP300. {ECO:0000269|PubMed:12569367, ECO:0000269|PubMed:12628924}.
Tissue SpecificityWidely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. {ECO:0000269|PubMed:9687510, ECO:0000269|PubMed:9873047}.
  logo