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MGP004223

UniProt Annotations

Entry Information

Gene Name	DLC1 Rho GTPase activating protein
Protein Entry	RHG07_HUMAN
UniProt ID	Q96QB1
Species	Human

Comments

Comment type	Description
Alternative Products	Event=Alternative promoter usage, Alternative splicing; Named isoforms=6; Name=2; IsoId=Q96QB1-2; Sequence=Displayed; Name=1; IsoId=Q96QB1-1; Sequence=VSP_037871, VSP_037872; Name=3; IsoId=Q96QB1-3; Sequence=VSP_037873, VSP_037874; Name=4; IsoId=Q96QB1-4; Sequence=VSP_037871, VSP_044651; Note=No experimental confirmation available.; Name=5; IsoId=Q96QB1-5; Sequence=VSP_046331, VSP_046332; Note=No experimental confirmation available.; Name=6; Synonyms=i-4; IsoId=Q96QB1-6; Sequence=VSP_053836, VSP_053837; Note=Produced by alternative promoter usage. ubiquitously expressed, significantly down-regulated in multiple carcinoma cell lines. Ref.8 (ABX83661/ABX83662) sequences are in conflict in positions: 49-50:EA->KP.;
Domain	The polybasic cluster is required for activation and mediates binding to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P(2)) containing membranes. {ECO:0000269\|PubMed:18786931}.
Domain	The SAM domain mediates interaction with EF1A1, and functions as an autoinhibitory regulator of RhoGAP Activity. {ECO:0000269\|PubMed:18786931}.
Function	Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality. {ECO:0000269\|PubMed:18786931, ECO:0000269\|PubMed:19170769, ECO:0000269\|PubMed:19710422}.
Interaction	P20936:RASA1; NbExp=6; IntAct=EBI-2608428, EBI-1026476;
Sequence Caution	Sequence=AAB81637.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAB21814.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Similarity	Contains 1 Rho-GAP domain. {ECO:0000255\|PROSITE- ProRule:PRU00172}.
Similarity	Contains 1 SAM (sterile alpha motif) domain. {ECO:0000305}.
Similarity	Contains 1 START domain. {ECO:0000255\|PROSITE- ProRule:PRU00197}.
Subcellular Location	Cytoplasm. Cell junction, focal adhesion. Membrane; Peripheral membrane protein. Note=Colocalizes with EF1A1 at actin-rich regions in the cell periphery.
Subunit	Interacts with EF1A1, facilitates EF1A1 distribution to the membrane periphery and ruffles upon growth factor stimulation and suppresses cell migration. {ECO:0000269\|PubMed:19158340}.
Tissue Specificity	Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.
Web Resource	Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/DLC1ID40328ch8p22.html";