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MGP004228

UniProt Annotations

Entry Information

Gene Name	NDC80 kinetochore complex component
Protein Entry	NDC80_HUMAN
UniProt ID	O14777
Species	Human

Comments

Comment type	Description
Developmental Stage	Expression peaks in mitosis. {ECO:0000269\|PubMed:16732327, ECO:0000269\|PubMed:9295362, ECO:0000269\|PubMed:9315664}.
Function	Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. {ECO:0000269\|PubMed:12351790, ECO:0000269\|PubMed:14654001, ECO:0000269\|PubMed:14699129, ECO:0000269\|PubMed:15062103, ECO:0000269\|PubMed:15235793, ECO:0000269\|PubMed:15239953, ECO:0000269\|PubMed:15548592, ECO:0000269\|PubMed:16732327, ECO:0000269\|PubMed:9315664}.
Interaction	Q9H3R5:CENPH; NbExp=3; IntAct=EBI-715849, EBI-1003700; Q9BZD4:NUF2; NbExp=10; IntAct=EBI-715849, EBI-724102; Q9HBM1:SPC25; NbExp=9; IntAct=EBI-715849, EBI-999909; O95229:ZWINT; NbExp=11; IntAct=EBI-715849, EBI-1001132;
Ptm	Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB. {ECO:0000269\|PubMed:12386167, ECO:0000269\|PubMed:14602875, ECO:0000269\|PubMed:20068231}.
Similarity	Belongs to the NDC80/HEC1 family. {ECO:0000305}.
Subcellular Location	Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
Subunit	Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24- SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C- terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. Interacts with isoform 1 of NEK2 and ZWINT specifically during mitosis. Interacts with CENPH and MIS12. May interact with AURKB, PSMC2, PSMC5 and SMC1A. May interact with RB1 during G2 phase and mitosis. {ECO:0000269\|PubMed:10409732, ECO:0000269\|PubMed:10779342, ECO:0000269\|PubMed:12386167, ECO:0000269\|PubMed:14602875, ECO:0000269\|PubMed:14699129, ECO:0000269\|PubMed:15502821, ECO:0000269\|PubMed:15713649, ECO:0000269\|PubMed:15824131, ECO:0000269\|PubMed:16732327, ECO:0000269\|PubMed:9295362}.