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MGP004402

UniProt Annotations

Entry Information
Gene Nameeuchromatic histone-lysine N-methyltransferase 2
Protein EntryEHMT2_HUMAN
UniProt IDQ96KQ7
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Comment=Additional isoforms seem to exist.; Name=1; IsoId=Q96KQ7-1; Sequence=Displayed; Name=2; Synonyms=NG36G9a-SPI; IsoId=Q96KQ7-2; Sequence=VSP_002211; Name=3; Synonyms=NG36; IsoId=Q96KQ7-3; Sequence=VSP_002212, VSP_002213;
Catalytic ActivityS-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. {ECO:0000269|PubMed:11316813, ECO:0000269|PubMed:20084102}.
CautionIt is uncertain whether Met-1 or Met-21 is the initiator methionine. {ECO:0000305}.
CautionWhile NG36 and G9a were originally thought to derive from 2 separate genes, all G9A transcripts also contain the in frame coding sequence of NG36. {ECO:0000305|PubMed:11707778}.
DomainIn the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster. {ECO:0000269|PubMed:18264113}.
DomainThe ANK repeats bind H3K9me1 and H3K9me2. {ECO:0000269|PubMed:18264113}.
DomainThe SET domain mediates interaction with WIZ. {ECO:0000269|PubMed:18264113}.
FunctionHistone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. {ECO:0000269|PubMed:11316813, ECO:0000269|PubMed:18438403, ECO:0000269|PubMed:20084102, ECO:0000269|PubMed:20118233, ECO:0000269|PubMed:22387026, ECO:0000269|PubMed:8457211}.
InteractionQ99684:GFI1; NbExp=2; IntAct=EBI-744366, EBI-949368; Q07120:Gfi1 (xeno); NbExp=3; IntAct=EBI-744366, EBI-4289236; Q13547:HDAC1; NbExp=3; IntAct=EBI-744366, EBI-301834; Q92831:KAT2B; NbExp=3; IntAct=EBI-744366, EBI-477430; Q9NQX1:PRDM5; NbExp=3; IntAct=EBI-744366, EBI-4292031; Q5JSZ5:PRRC2B; NbExp=2; IntAct=EBI-744366, EBI-744891; Q9P2R6:RERE; NbExp=3; IntAct=EBI-744366, EBI-948076;
PtmMethylated at Lys-185; automethylated. {ECO:0000269|PubMed:18438403}.
Sequence CautionSequence=AAD21811.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=AAD21812.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=AAH02686.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=AAH09351.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=AAH18718.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH20970.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=BAB63294.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=BAB63295.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAA49491.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
SimilarityBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily. {ECO:0000255|PROSITE- ProRule:PRU00190}.
SimilarityContains 1 post-SET domain. {ECO:0000305}.
SimilarityContains 1 pre-SET domain. {ECO:0000255|PROSITE- ProRule:PRU00157}.
SimilarityContains 1 SET domain. {ECO:0000255|PROSITE- ProRule:PRU00190}.
SimilarityContains 7 ANK repeats. {ECO:0000255|PROSITE- ProRule:PRU00023}.
Subcellular LocationNucleus {ECO:0000269|PubMed:11316813}. Chromosome {ECO:0000269|PubMed:11316813}. Note=Associates with euchromatic regions. Does not associate with heterochromatin.
SubunitHeterodimer; heterodimerizes with EHMT1/GLP. Interacts with GFI1B and WIZ. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with UHRF1. Interacts with CDYL. Interacts with REST only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2. {ECO:0000269|PubMed:12004135, ECO:0000269|PubMed:16688220, ECO:0000269|PubMed:16702210, ECO:0000269|PubMed:19056828, ECO:0000269|PubMed:19061646, ECO:0000269|PubMed:20084102, ECO:0000269|PubMed:20118233, ECO:0000269|PubMed:21549307}.
Tissue SpecificityExpressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. {ECO:0000269|PubMed:11707778}.
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