MGP Database

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MGP004502

UniProt Annotations

Entry Information
Gene Namecytochrome P450, family 4, subfamily F, polypeptide 8
Protein EntryCP4F8_HUMAN
UniProt IDP98187
SpeciesHuman
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=7 uM for 9-alpha,11-alpha-epoxymethano-PGH2 (U-44069); KM=40 uM for 6,9-alpha-methylene-PGI2; Note=The Vmax of the reaction with U-44069 as substrate is 260 pmol/min/pmol enzyme.;
Catalytic ActivityRH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.
CofactorName=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
FunctionHydroxylates arachidonic acid (20:4n-6) to (18R)- hydroxyarachidonate. Shows little activity against prostaglandin (PG) D2, PGE1, PGE2, PGF2alpha, and leukotriene B4. Catalyzes omega-2 and omega-3-hydroxylation of PGH1 and PGH2. Catalyzes epoxidation of 4,7,10,13,16,19-(Z)-docosahexaenoic acid (22:6n-3) and 7,10,13,16,19-(Z)-docosapentaenoic acid (22:5n-3) and omega-3- hydroxylation of 4,7,10,13,16-(Z)-docosapentaenoic acid (22:5n-6). Catalyzes hydroxylation of PGI2 and carbaprostacyclin. {ECO:0000269|PubMed:10791960, ECO:0000269|PubMed:15789615, ECO:0000269|PubMed:16112640}.
SimilarityBelongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Microsome membrane {ECO:0000250}.
Tissue SpecificityExpressed in the epithelium of seminal vesicles, in renal cortex, in adult and fetal liver, in epidermis, in corneal epithelium, in sweat glands, hair follicles, epithelial linings of the ampulla of vas deferens and of the stomach and small intestine, as well as in the transitional epithelium of the bladder and ureter (at protein level). In the epidermis, expressed from the basal cell to the granular cell layers. In the corneal epithelium, expressed in all cell layers. Also detected in prostate. Up-regulated in the epidermis of psoriatic lesions. {ECO:0000269|PubMed:10405341, ECO:0000269|PubMed:12464258, ECO:0000269|PubMed:15789615}.
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