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MGP005172

UniProt Annotations

Entry Information
Gene Namecarbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
Protein EntryCHSTF_HUMAN
UniProt IDQ7LFX5
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q7LFX5-1; Sequence=Displayed; Name=2; IsoId=Q7LFX5-2; Sequence=VSP_017387; Note=No experimental confirmation available.;
Catalytic Activity3-phospho-5-adenylyl sulfate + [chondroitin]- 4-O-sulfo-N-acetylgalactosamine = adenosine 3',5'-bisphosphate + [chondroitin]-4,6-di-O-sulfo-N-acetyl-D-galactosamine. {ECO:0000269|PubMed:12874280}.
Catalytic Activity3-phospho-5-adenylyl sulfate + [dermatan]-4-O- sulfo-N-acetylgalactosamine = adenosine 3',5'-bisphosphate + [dermatan]-4,6-di-O-sulfo-N-acetyl-D-galactosamine. {ECO:0000269|PubMed:12874280}.
CofactorName=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Evidence={ECO:0000250};
CofactorName=glutathione; Xref=ChEBI:CHEBI:57925; Evidence={ECO:0000250};
Enzyme RegulationInhibited by phenyl beta-GalNAc(4,6-SO(4)). {ECO:0000269|PubMed:16024005}.
FunctionSulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. {ECO:0000269|PubMed:11572857, ECO:0000269|PubMed:12874280}.
PtmGlycosylated. {ECO:0000269|PubMed:10749872}.
Sequence CautionSequence=AAC71691.1; Type=Frameshift; Positions=180, 223, 489, 537; Evidence={ECO:0000305}; Sequence=AAH50540.1; Type=Frameshift; Positions=511; Evidence={ECO:0000305}; Sequence=BAA25524.2; Type=Erroneous initiation; Evidence={ECO:0000305};
SimilarityBelongs to the sulfotransferase 1 family. {ECO:0000305}.
Subcellular LocationGolgi apparatus membrane {ECO:0000305|PubMed:10749872}; Single-pass type II membrane protein {ECO:0000305|PubMed:10749872}. Note=A small fraction may also be present at the cell surface, where it acts as a B-cell receptor.
SubunitHomodimer; disulfide-linked (Potential). The relevance of homodimerization is however unsure. May interact with phosphorylated proteins in resting B-cells, including HCK. {ECO:0000269|PubMed:10749872, ECO:0000305}.
Tissue SpecificityExpressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. {ECO:0000269|PubMed:9754571}.
Web ResourceName=GGDB; Note=GlycoGene database; URL="http://jcggdb.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=CHST15";
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