MGP Database

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MGP006168

UniProt Annotations

Entry Information
Gene Namecarnosine dipeptidase 1 (metallopeptidase M20 family)
Protein EntryCNDP1_HUMAN
UniProt IDQ96KN2
SpeciesHuman
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=1.27 uM for carnosine (at 30 degrees Celsius and in the absence of cadmium ions) {ECO:0000269|PubMed:12473676}; KM=11.00 uM for carnosine (at 30 degrees Celsius and in the presence of 200 uM cadmium ions) {ECO:0000269|PubMed:12473676}; KM=0.20 uM for homocarnosine (at 30 degrees Celsius and in the absence of cadmium ions) {ECO:0000269|PubMed:12473676}; KM=1.0 uM for homocarnosine (at 30 degrees Celsius and in the presence of 200 uM cadmium ions) {ECO:0000269|PubMed:12473676}; Note=1 hour incubation in 50 mM Tris-HCl, pH 7.5.; pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:12473676};
Catalytic ActivityPreferential hydrolysis of the beta-Ala-|-His dipeptide (carnosine), and also anserine, Xaa-|-His dipeptides and other dipeptides including homocarnosine. {ECO:0000269|PubMed:12473676, ECO:0000269|PubMed:7116644}.
CofactorName=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|Ref.10}; Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000269|Ref.10};
Enzyme RegulationInhibited by the metal chelator 1,10-o- phenantrolin. The inhibitory concentration 50% (IC(50)) is 5 uM.
PolymorphismThe number of trinucleotide (CTG) repeat varies among different alleles leading to insertion of Leu residues in the signal peptide. The allele with 5 leucines (as shown in the reference entry) is known as the Mannheim allele. Diabetic patients with the CNDP1 Mannheim allele are less susceptible for nephropathy.
SimilarityBelongs to the peptidase M20A family. {ECO:0000305}.
Subcellular LocationSecreted.
SubunitHomodimer. {ECO:0000269|PubMed:7116644, ECO:0000269|Ref.10}.
Tissue SpecificityFound in serum and adult nervous central system. Absent in serum from patients with homocarnosinosis. {ECO:0000269|PubMed:12473676, ECO:0000269|PubMed:6616870}.
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