MGP Database

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MGP007013

UniProt Annotations

Entry Information
Gene Namealkaline ceramidase 2
Protein EntryACER2_HUMAN
UniProt IDQ5QJU3
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q5QJU3-1; Sequence=Displayed; Name=2; IsoId=Q5QJU3-2; Sequence=VSP_020033; Name=3; IsoId=Q5QJU3-3; Sequence=VSP_020033, VSP_020034, VSP_020035; Note=No experimental confirmation available.;
Biophysicochemical PropertiesKinetic parameters: KM=81 uM for D-erythro-C(24:1)-ceramide (at 37 degrees Celsius and pH 9.0) {ECO:0000269|PubMed:20089856}; Vmax=27 pmol/min/mg enzyme with D-erythro-C(24:1)-ceramide as substrate {ECO:0000269|PubMed:20089856}; pH dependence: Optimum pH is 7.5-9.0. {ECO:0000269|PubMed:20089856};
Catalytic ActivityN-acylsphingosine + H(2)O = a carboxylate + sphingosine.
Enzyme RegulationSpecifically activated by lumenal, but not cytosolic Ca(2+). Inhibited by Zn(2+) or Cu(2+). Mg(2+) or Mn(2+) have no effect on ceramidase activity. {ECO:0000269|PubMed:20089856}.
FunctionHydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)- ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)- ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate. {ECO:0000269|PubMed:16940153, ECO:0000269|PubMed:18945876, ECO:0000269|PubMed:20089856}.
SimilarityBelongs to the alkaline ceramidase family. {ECO:0000305}.
Subcellular LocationGolgi apparatus membrane {ECO:0000269|PubMed:16940153, ECO:0000269|PubMed:20089856}; Multi- pass membrane protein {ECO:0000269|PubMed:16940153, ECO:0000269|PubMed:20089856}.
Tissue SpecificityHighly expressed in placenta. {ECO:0000269|PubMed:16940153}.
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