Summary of Study ST001032
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000690. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ3M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001032 |
Study Title | Single-cell Profiling of Cationic and Anionic Metabolites in Live Frog (Xenopus) Embryos using Microprobe Capillary Electrophoresis Mass Spectrometry |
Study Type | Metabolic profiling of single cells |
Study Summary | The goal of this study was to enable the analysis of anionic and cationic metabolites from the same identified single cell in Xenopus laevis embryos. A 10 nL portion of identified animal-ventral (V1) cells was aspirated from 8-cell embryos, and metabolites were extracted from the aspirate, before characterization of cationic and anionic compounds using a custom-built capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry platform. A total of ~250 cationic molecular features and ~150 anionic molecular features were detected, including 76 metabolites that were identified in this study. Pathway analysis of the identified metabolites highlighted arginine-proline metabolism of significance. |
Institute | University of Maryland |
Department | Department of Chemistry & Biochemistry |
Laboratory | Nemes Laboratory |
Last Name | Nemes |
First Name | Peter |
Address | 0107 Chemistry Building 8051 Regents Drive |
nemes@umd.edu | |
Phone | 3014050373 |
Submit Date | 2018-08-08 |
Num Groups | 4 biological replicates (each different cell from a different embryo) + 1-to-2 technical replicates (same extract analyzed multiple times) |
Total Subjects | 4 different V1 cells were analyzed, each from a different embryo |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2019-09-23 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001692 | AN001693 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | CE | CE |
Chromatography system | Custom-built CE system | Custom-built CE system |
Column | Bare fused silica capillary | Bare fused silica capillary |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Bruker Impact HD | Bruker Impact HD |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |