Summary of Study ST002055
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001300. The data can be accessed directly via it's Project DOI: 10.21228/M8P69K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002055 |
Study Title | Metabolomic Profiling of Human Pluripotent Stem Cell Differentiation into Lung Progenitors |
Study Summary | Metabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation towards a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification towards lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in fetal lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development. |
Institute | The Hospital for Sick Children |
Last Name | Post |
First Name | Martin |
Address | 555 University Avenue |
martin.post@sickkids.ca | |
Phone | 4168136772 |
Submit Date | 2021-06-29 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS/MS(Dir. Inf.) |
Release Date | 2022-01-13 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN003346 | AN003347 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | None (Direct infusion) |
Chromatography system | Agilent 1290 Infinity | - |
Column | Agilent Eclipse XDB-C18 (100 x 3.0mm) | - |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | POSITIVE | UNSPECIFIED |
Units | uM | uM |
Chromatography:
Chromatography ID: | CH002477 |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Agilent Eclipse XDB-C18 (100 x 3.0mm) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH002478 |
Instrument Name: | - |
Column Name: | - |
Chromatography Type: | None (Direct infusion) |