Summary of Study ST002745
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002745 |
Study Title | Biomolecular condensates create phospholipid-enriched microenvironments (Part 6) |
Study Type | Metabolomes of in vitro synthesized condensates |
Study Summary | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. Here, we quantified the concentration of a select set of phospholipids in the aqueous and condensate phase of condensates formed from the low complexity domain of MED1 by comparison with isotopic-labeled phospholipid standards. |
Institute | Cornell University |
Department | Department of Pharmacology |
Laboratory | Dr. Samie Jaffrey |
Last Name | Dumelie |
First Name | Jason |
Address | 1300 York Ave, LC-524, New York City, NY |
srj2003@med.cornell.edu | |
Phone | 6465690174 |
Submit Date | 2023-06-15 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzdata.xml |
Analysis Type Detail | LC-MS |
Release Date | 2023-07-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004451 | AN004452 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent Model 1290 Infinity II liquid chromatography system | Agilent Model 1290 Infinity II liquid chromatography system |
Column | Cogent Diamond Hydride (150 × 2.1 mm, 4um) | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6460 QQQ | Agilent 6460 QQQ |
Ion Mode | POSITIVE | POSITIVE |
Units | Ion abundance (peak area) | Ion abundance (peak area) |
Chromatography:
Chromatography ID: | CH003343 |
Chromatography Summary: | Chromatography of metabolites utilized reversed phase chromatography on a Agilent ZORBAX Eclipse Plus C18, 100 × 2.1 mm, 1.8 μm. Mobile phases consisted of (A) 10 mM ammonium formate with 5 μM Agilent deactivator additive in 5:3:2 water:acetonitrile:2-propanol and (B) 10 mM ammonium formate in 1:9:90 water:acetonitrile:2-propanol. Column temperature was set at 60°C and autosampler temperature was at 20°C. The flow rate was 0.4 mL/min. The following gradient was applied: 0 min, 15% B; 0-2.5 min, to 50% B; 2.5-2.6 min, to 57%, 2.6-9 min, to 70% B; 9-9.1 min, to 93% B; 9.1-11.1 min, to 96%; 11.1- 15min, 100% B; 15-20 min, 15% B. |
Instrument Name: | Agilent Model 1290 Infinity II liquid chromatography system |
Column Name: | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
Column Temperature: | 60 |
Flow Gradient: | 0 min, 15% B; 0-2.5 min, to 50% B; 2.5-2.6 min, to 57%, 2.6-9 min, to 70% B; 9-9.1 min, to 93% B; 9.1-11.1 min, to 96%; 11.1- 15min, 100% B; 15-20 min, 15% B. |
Flow Rate: | 0.4 mL/min |
Solvent A: | 50% water/30% acetonitrile/20% isopropanol;10 mM ammonium formate with 5 µM Agilent deactivator additive |
Solvent B: | 1% water/9% acetonitrile/90% isopropanol;10 mM ammonium formate |
Chromatography Type: | Reversed phase |