Summary of Study ST002745
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002745 |
| Study Title | Biomolecular condensates create phospholipid-enriched microenvironments (Part 6) |
| Study Type | Metabolomes of in vitro synthesized condensates |
| Study Summary | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. Here, we quantified the concentration of a select set of phospholipids in the aqueous and condensate phase of condensates formed from the low complexity domain of MED1 by comparison with isotopic-labeled phospholipid standards. |
| Institute | Cornell University |
| Department | Department of Pharmacology |
| Laboratory | Dr. Samie Jaffrey |
| Last Name | Dumelie |
| First Name | Jason |
| Address | 1300 York Ave, LC-524, New York City, NY |
| srj2003@med.cornell.edu | |
| Phone | 6465690174 |
| Submit Date | 2023-06-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzdata.xml |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004451 | AN004452 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent Model 1290 Infinity II liquid chromatography system | Agilent Model 1290 Infinity II liquid chromatography system |
| Column | Cogent Diamond Hydride (150 × 2.1 mm, 4um) | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6460 QQQ | Agilent 6460 QQQ |
| Ion Mode | POSITIVE | POSITIVE |
| Units | Ion abundance (peak area) | Ion abundance (peak area) |
MS:
| MS ID: | MS004198 |
| Analysis ID: | AN004451 |
| Instrument Name: | Agilent 6460 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | (PE, PC, SM, others)LC/MS-based targeted metabolite profiling. MS mode was a Agilent Jet Stream ESI. To quantify phospholipid concentrations, we used stable isotope standards and dynamic multiple reaction monitoring (dMRM). The transitions for lipid standards were monitored as recommended by the supplier. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004199 |
| Analysis ID: | AN004452 |
| Instrument Name: | Agilent 6460 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | (PI) LC/MS-based targeted metabolite profiling. MS mode was a Agilent Jet Stream ESI. To quantify phospholipid concentrations, we used stable isotope standards and dynamic multiple reaction monitoring (dMRM). The transitions for lipid standards were monitored as recommended by the supplier. |
| Ion Mode: | POSITIVE |
