Summary of Study ST002745
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002745 |
| Study Title | Biomolecular condensates create phospholipid-enriched microenvironments (Part 6) |
| Study Type | Metabolomes of in vitro synthesized condensates |
| Study Summary | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. Here, we quantified the concentration of a select set of phospholipids in the aqueous and condensate phase of condensates formed from the low complexity domain of MED1 by comparison with isotopic-labeled phospholipid standards. |
| Institute | Cornell University |
| Department | Department of Pharmacology |
| Laboratory | Dr. Samie Jaffrey |
| Last Name | Dumelie |
| First Name | Jason |
| Address | 1300 York Ave, LC-524, New York City, NY |
| srj2003@med.cornell.edu | |
| Phone | 6465690174 |
| Submit Date | 2023-06-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzdata.xml |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-07 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002858 |
| Sampleprep Summary: | Dried-down extracts were reconstituted in 150 µl 70% acetonitrile, at a relative protein concentration of ~ 2 µg/µl. These were stored until used at -20C. SPLASH® LIPIDOMIX® Quantitative Mass Spec Standard was diluted 1/30 or 1/200, combined with the reconstituted extract and then either 3 µl (for PIs samples) or 5 µl (all other samples) were injected for LC/MS-based targeted metabolite profiling. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -20℃ |
