Summary of Study ST002745
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002745 |
| Study Title | Biomolecular condensates create phospholipid-enriched microenvironments (Part 6) |
| Study Type | Metabolomes of in vitro synthesized condensates |
| Study Summary | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. Here, we quantified the concentration of a select set of phospholipids in the aqueous and condensate phase of condensates formed from the low complexity domain of MED1 by comparison with isotopic-labeled phospholipid standards. |
| Institute | Cornell University |
| Department | Department of Pharmacology |
| Laboratory | Dr. Samie Jaffrey |
| Last Name | Dumelie |
| First Name | Jason |
| Address | 1300 York Ave, LC-524, New York City, NY |
| srj2003@med.cornell.edu | |
| Phone | 6465690174 |
| Submit Date | 2023-06-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzdata.xml |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-07-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | fraction | Extraction from condensate |
|---|---|---|---|
| SA289178 | MED1 PE Aqueous Sample 5 | aqueous | No heat step |
| SA289179 | MED1 PO Aqueous Sample 5 | aqueous | No heat step |
| SA289180 | MED1 PI Aqueous Sample 6 | aqueous | No heat step |
| SA289181 | MED1 PO Aqueous Sample 6 | aqueous | No heat step |
| SA289182 | MED1 PI Aqueous Sample 5 | aqueous | No heat step |
| SA289183 | MED1 PE Aqueous Sample 6 | aqueous | No heat step |
| SA289184 | MED1 PI Aqueous Sample 9 | aqueous | standard |
| SA289185 | MED1 PE Aqueous Sample 9 | aqueous | standard |
| SA289186 | MED1 PO Aqueous Sample 9 | aqueous | standard |
| SA289187 | MED1 PO Condensate Sample 5 | condensate | No heat step |
| SA289188 | MED1 PO Condensate Sample 6 | condensate | No heat step |
| SA289189 | MED1 PI Condensate Sample 6 | condensate | No heat step |
| SA289190 | MED1 PI Condensate Sample 5 | condensate | No heat step |
| SA289191 | MED1 PE Condensate Sample 6 | condensate | No heat step |
| SA289192 | MED1 PE Condensate Sample 5 | condensate | No heat step |
| SA289193 | MED1 PO Condensate Sample 9 | condensate | standard |
| SA289194 | MED1 PI Condensate Sample 9 | condensate | standard |
| SA289195 | MED1 PE Condensate Sample 9 | condensate | standard |
| SA289196 | MED1 PO Input Sample 6 | input | No heat step |
| SA289197 | MED1 PO Input Sample 5 | input | No heat step |
| SA289198 | MED1 PE Input Sample 5 | input | No heat step |
| SA289199 | MED1 PI Input Sample 6 | input | No heat step |
| SA289200 | MED1 PE Input Sample 6 | input | No heat step |
| SA289201 | MED1 PI Input Sample 5 | input | No heat step |
| SA289202 | MED1 PO Input Sample 9 | input | standard |
| SA289203 | MED1 PI Input Sample 9 | input | standard |
| SA289204 | MED1 PE Input Sample 9 | input | standard |
| Showing results 1 to 27 of 27 |
