Summary of Study ST003047
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001897. The data can be accessed directly via it's Project DOI: 10.21228/M8HH8R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003047 |
Study Title | Defective mitochondria remodelling in B cells leads to an aged immune response |
Study Summary | The germinal centre (GC) reaction requires a unique bioenergetic supply. Although mitochondria are remodelled upon antigen stimulation, mitochondrial function in B cells is still poorly understood. To gain a better understanding of the role of mitochondria in B cell function, we generated mice that lack, specifically in B cells, Tfam, a transcription factor necessary for mitochondrial biogenesis. Tfam knock-out (KO) mice displayed a blockage of the GC reaction and established an immune response featured by the differentiation of activated B cells towards memory B cells and aged-related B cells, hallmarks of an aged immune response. Unexpectedly, GC blockage in Tfam KO mice did not cause defects in the bioenergetic supply, but this phenotype was associated with a defect in the remodelling of the lysosomal compartment in B cells. Therefore, these results may describe a new mitochondrial function for antigen presentation during the GC reaction, the abrogation of which may be the basis of an aged immune response. |
Institute | Consejo Superior de Investigaciones Científicas |
Last Name | Martínez |
First Name | Nuria |
Address | Calle Nicolás Cabrera, 1, Madrid, Madrid, 28049, Spain |
nmartinez@cbm.csic.es | |
Phone | 0034-911964517 |
Submit Date | 2024-01-17 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS |
Release Date | 2024-02-05 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004998 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 8890 |
Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5977B |
Ion Mode | POSITIVE |
Units | relative abundance |
Chromatography:
Chromatography ID: | CH003776 |
Chromatography Summary: | For GC-MS, the samples were injected onto a DB5-MS column (30 m x 0.250 mm, 0.25 µm) with a 10 m pre-column (Agilent Technologies, Santa Clara CA, USA). Helium was used as mobile phase at constant flow rate (1 mL/min) in a GC-Q-MS (8890 GC coupled to 5977B MS) system (Agilent Technologies) with an Electron Ionization (EI) source at 70 eV. |
Methods Filename: | Protocol_BL.pdf |
Instrument Name: | Agilent 8890 |
Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
Column Temperature: | -60 °C-325/350 °C |
Flow Gradient: | NA |
Flow Rate: | 1 mL/min |
Solvent A: | NA |
Solvent B: | NA |
Chromatography Type: | GC |