Summary of Study ST003047
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001897. The data can be accessed directly via it's Project DOI: 10.21228/M8HH8R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003047 |
Study Title | Defective mitochondria remodelling in B cells leads to an aged immune response |
Study Summary | The germinal centre (GC) reaction requires a unique bioenergetic supply. Although mitochondria are remodelled upon antigen stimulation, mitochondrial function in B cells is still poorly understood. To gain a better understanding of the role of mitochondria in B cell function, we generated mice that lack, specifically in B cells, Tfam, a transcription factor necessary for mitochondrial biogenesis. Tfam knock-out (KO) mice displayed a blockage of the GC reaction and established an immune response featured by the differentiation of activated B cells towards memory B cells and aged-related B cells, hallmarks of an aged immune response. Unexpectedly, GC blockage in Tfam KO mice did not cause defects in the bioenergetic supply, but this phenotype was associated with a defect in the remodelling of the lysosomal compartment in B cells. Therefore, these results may describe a new mitochondrial function for antigen presentation during the GC reaction, the abrogation of which may be the basis of an aged immune response. |
Institute | Consejo Superior de Investigaciones Científicas |
Last Name | Martínez |
First Name | Nuria |
Address | Calle Nicolás Cabrera, 1, Madrid, Madrid, 28049, Spain |
nmartinez@cbm.csic.es | |
Phone | 0034-911964517 |
Submit Date | 2024-01-17 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS |
Release Date | 2024-02-05 |
Release Version | 1 |
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Collection:
Collection ID: | CO003155 |
Collection Summary: | Tfamfl/fl mice (Larsson et al., 1998) were provided by Larsson NG (Max Plack Institute for Biology of Ageing, Cologne, Germany) and were crossed at the Centro de Biologia Molecular Severo Ochoa (CBMSO) animal facility with MB1Cre mice (Hobeika et al., 2006). MB1Cre mice were kindly provided by Alarcón B (CBMSO, Madrid, Spain). Mouse colonies were bred at the CBMSO under specific pathogen-free conditions and on C57BL/6 background. Mice were group-housed, have not been used in previous procedures and were fed with standard chow. Littermates were randomly assigned to experimental groups. Male and female between the ages of 8-16 weeks were used for the experiment. Splenic naive B lymphocytes were purified using negative B cell cell isolation kits (Miltenyi Biotec). After 24 hours stimulation, live cells were sorted, pelleted and frozen with liquid N2. |
Collection Protocol Filename: | Protocol_BL.pdf |
Sample Type: | B-cells |