Summary of Study ST003047
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001897. The data can be accessed directly via it's Project DOI: 10.21228/M8HH8R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003047 |
Study Title | Defective mitochondria remodelling in B cells leads to an aged immune response |
Study Summary | The germinal centre (GC) reaction requires a unique bioenergetic supply. Although mitochondria are remodelled upon antigen stimulation, mitochondrial function in B cells is still poorly understood. To gain a better understanding of the role of mitochondria in B cell function, we generated mice that lack, specifically in B cells, Tfam, a transcription factor necessary for mitochondrial biogenesis. Tfam knock-out (KO) mice displayed a blockage of the GC reaction and established an immune response featured by the differentiation of activated B cells towards memory B cells and aged-related B cells, hallmarks of an aged immune response. Unexpectedly, GC blockage in Tfam KO mice did not cause defects in the bioenergetic supply, but this phenotype was associated with a defect in the remodelling of the lysosomal compartment in B cells. Therefore, these results may describe a new mitochondrial function for antigen presentation during the GC reaction, the abrogation of which may be the basis of an aged immune response. |
Institute | Consejo Superior de Investigaciones Científicas |
Last Name | Martínez |
First Name | Nuria |
Address | Calle Nicolás Cabrera, 1, Madrid, Madrid, 28049, Spain |
nmartinez@cbm.csic.es | |
Phone | 0034-911964517 |
Submit Date | 2024-01-17 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS |
Release Date | 2024-02-05 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR003171 |
Treatment Summary: | Mice were group-housed and fed with standard chow. B lympchocytes from wild-type and knock-out mice were isolated and cultured for 24 hours in RPMI 1640 supplemented with 10% FCS, 1mM Glutamine, 0,01% sodium pyruvate, 20mM 2-mercaptoethanol, penicillin and streptomycin. Cells were cultured adding interleukine-4 and interleukine-5 at 10 ng/Ml, anti-IgM 1 ug/mL and anti-CD40 0,3 ug/mL. |