Summary of Study ST003047
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001897. The data can be accessed directly via it's Project DOI: 10.21228/M8HH8R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003047 |
| Study Title | Defective mitochondria remodelling in B cells leads to an aged immune response |
| Study Summary | The germinal centre (GC) reaction requires a unique bioenergetic supply. Although mitochondria are remodelled upon antigen stimulation, mitochondrial function in B cells is still poorly understood. To gain a better understanding of the role of mitochondria in B cell function, we generated mice that lack, specifically in B cells, Tfam, a transcription factor necessary for mitochondrial biogenesis. Tfam knock-out (KO) mice displayed a blockage of the GC reaction and established an immune response featured by the differentiation of activated B cells towards memory B cells and aged-related B cells, hallmarks of an aged immune response. Unexpectedly, GC blockage in Tfam KO mice did not cause defects in the bioenergetic supply, but this phenotype was associated with a defect in the remodelling of the lysosomal compartment in B cells. Therefore, these results may describe a new mitochondrial function for antigen presentation during the GC reaction, the abrogation of which may be the basis of an aged immune response. |
| Institute | Consejo Superior de Investigaciones Científicas |
| Last Name | Martínez |
| First Name | Nuria |
| Address | Calle Nicolás Cabrera, 1, Madrid, Madrid, 28049, Spain |
| nmartinez@cbm.csic.es | |
| Phone | 0034-911964517 |
| Submit Date | 2024-01-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-02-05 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003168 |
| Sampleprep Summary: | From wild-type samples WT_A, WT_B and WT_C 5.6 x 106, 6.4 x 106 and 4.87 x 106 B lymphocytes were harvested respectively, and for knock-out samples KO_D, KO_E and KO_F 1.8 x 106, 2.1 x 106 and 1.8 x 106 B lymphocytes were harvested respectively. Cell homogenates were prepared by adding 1:1 v/v water: methanol and sonicated with a dr.hielscher UP200S (dr.hielscher, Berlin, Germany), set up to 80% intensity, 0.5 s/pulse, 16 pulses. For GC-MS, 100 µL of each homogenate was mixed with 300 µL of cold methanol containing 25 ppm D-palmitic acid (internal standard), vortex mixed for 60 min and centrifuged at 4000 g for 20 min. 250 µL of the supernatant were transferred to a vial with a glass insert and evaporated to dryness in a Gyrozen HyperVac VC2124 (Gimpo, Korea) coupled to a LVS 110Z (Gardner Denver Thomas GmbH Welch Vacuum, Fürstenfeldbruck, Germany). The samples were then submitted to methoximation (with O-methoxyamine) for 16 h and silylation for 1 h at 70ºC with N,O-Bis(trime5j,thylsilyl)trifluoroacetamide (BSTFA) and trimethylchlorosilane (TCMS), and finally resuspended in 100 µL heptane. |
| Sampleprep Protocol Filename: | Protocol_BL.pdf |
