Summary of Study ST002893
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001773. The data can be accessed directly via it's Project DOI: 10.21228/M8J420 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002893 |
Study Title | Folate depletion time-course in MEL cells with analysis for porphyrin metabolites |
Study Summary | Culture of MEL cells in RPMI media containing 100 nM folic acid for 0, 1, 2, 4, 6, or 8 days followed my LC-MS targeting porphyrin metabolites. This is a reverse timecourse where all samples are harvested on the same day. Day 0 in 100 nM folic acid indicates 8 days culture in 2,000 nM folic acid. |
Institute | Boston Children's Hospital, Harvard Medical School |
Department | pathology |
Laboratory | Kanarek Lab |
Last Name | Kanarek |
First Name | Naama |
Address | Enders 1116.2, 300 Longwood Ave, Boston, MA 02115 |
naama.kanarek@childrens.harvard.edu | |
Phone | 6173557433 |
Submit Date | 2023-09-28 |
Num Groups | 5 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-02-13 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004752 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Phenomenex Kinetex C18 (150 x 3mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | peak area |
MS:
MS ID: | MS004498 |
Analysis ID: | AN004752 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The mass spectrometer was operated in full-scan, positive ionization mode using a narrow-range scan: 450-700m/z, with an additional tSIM scan for hemin (616.1767 m/z), CoproP (655.2762 m/z), and PPIX (563.2653 m/z). Metabolites were relatively quantified while referencing an in-house library of chemical standards and using TraceFinder 4.1 (Thermo Fisher Scientific, Waltham, MA, USA), with a 5 ppm mass tolerance. |
Ion Mode: | POSITIVE |