Summary of Study ST001260
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000846. The data can be accessed directly via it's Project DOI: 10.21228/M8B97R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001260 |
Study Title | Metabolic changes of Fusobacterium nucleatum when co-cultured with other oral microbes (part-I) |
Study Summary | We used membrane-separated co-culture systems to globally assess metabolomic changes of Fusobacterium nucleatum when co-cultured with Streptococcus gordonii and/or Veillonella parvula. |
Institute | Osaka University |
Department | Graduate School of Dentistry, Department of Preventive Dentistry |
Last Name | Kuboniwa |
First Name | Masae |
Address | Yamadaoka 1-8 |
kuboniwa@dent.osaka-u.ac.jp | |
Phone | 81668792922 |
Submit Date | 2019-09-26 |
Analysis Type Detail | LC-MS |
Release Date | 2021-04-01 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001336 |
Sampleprep Summary: | To remove protein, 2 ml of chloroform and 0.8 ml of ultrapure water were added to the samples, which were thoroughly mixed and centrifuged at 2300 × g for 5 minutes at 4˚C. The upper aqueous layer was then transferred to ultrafilter tips (Amicon ultrafilter system™) and centrifuged at 9100 × g for 120 minutes at 4˚C. Filtered material was dried under reduced pressure, followed by suspension in 50 µl of ultrapure water. |